COURSE SYLLABUS

ADVANCED SEMEN ANALYSIS TECHNIQUES

1	Course Title:	ADVANCED SEMEN ANALYSIS TECHNIQUES
2	Course Code:	VDT6018
3	Type of Course:	Optional
4	Level of Course:	Third Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	5
8	Theoretical (hour/week):	2
9	Practice (hour/week) :	2
10	Laboratory (hour/week) :	0
11	Prerequisites:
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Doç. Dr. BURCU ÜSTÜNER
16	Course Lecturers:
17	Contactinformation of the Course Coordinator:	bbaspinar@uludag.edu.tr, 224-2941245 Uludağ Üniversitesi Veteriner Fakültesi Dölerme ve Suni Tohumlama AD
18	Website:	http://saglikbilimleri.uludag.edu.tr/anabilimdallari.php
19	Objective of the Course:	Semen characteristics, which has an important in determining male infertility and nowadays specific examination techniques used in andrology laboratory are intended to reveal.
20	Contribution of the Course to Professional Development

Learning Outcomes:

1	To have information about the structure of spermatozoa ultrastructure. ;
2	The evaluation and interpretation of plasma membrane, acrosome, mitochondria and DNA integrity with fluorescent dyes;
3	Being knowledgeable about sperm separation technique used in assisted reproduction techniques of cattle;
4	Being knowledgeable about sperm separation technique used in assisted reproduction techniques of sheep;
5	Being knowledgeable about sperm function tests;
6	The evaluation of adverse effects of freezing procedures on spermatozoon ;
7	Being knowledgeable about oxidative stress and sperm;
8	Lyophilized sperm and assisted reproductive techniques;

22	Course Content:

Week	Theoretical	Practical
1	The importance of semen characteristics and the relationship between fertility	The introduction of the andrology laboratory
2	Spermatogenesis and ultrastructural evaluation of semen	Semen collection and evaluation of mass activity, motility and sperm concentration.
3	Sperm washing for analyse and Assisted Reproductive Technologies (ART)	Semen preparation for in vitro fertilization.
4	Routine semen analysis used in andrology laboratory	Determination of sperm acrosome and morphological integrity.
5	Evaluation and importance of sperm plasma membrane	Determination of sperm plasma integrity with fluorescent dyes
6	Evaluation and importance of sperm DNA integrity	Determination of sperm DNA integrity with fluorescent dyes
7	Evaluation and importance of sperm acrosomal membrane	Determination of sperm acrosomal integrity with fluorescent dyes
8	Evaluation and importance of sperm mitochondrial activity	Determination of sperm mitochondrial membrane integrity with fluorescent dyes
9	Evaluation of sperm kinematics with CASA (Computer-assisted semen analysis)	Evaluation of lyophilized spermatological characteristics
10	Functional tests associated with fertility	ICSI application with lyophilized semen
11	Effects of extenders which prepared for semen freezing on spermatological parameters	Determination of adverse effects of freezing diluents on spermatological characteristics
12	The effect of oxidative stress in semen	Interpretation of oxidative stress parameters in semen
13	Destruction of plasma membrane with Triton X-100 and Lysolecithin	Destruction of plasma membrane with Triton X-100 and Lysolecithin and assessment with fluorescent microscope
14	Induction of sperm capacitation and acrosome reaction with heparin and Ca-ionophore	Induction of sperm capacitation and acrosome reaction with heparin and Ca-ionophore and assessment with fluorescent microscope

23	Textbooks, References and/or Other Materials:	NOAKES, D.E., PARKINSON, T.J., ENGLAND, G.C.W., Arthur’s Veterinary Reproduction and Obstetrics, Saunders Inc., New York, USA GORDON, I., Laboratory Production of Cattle Embryos, 2nd edition. CABI Publisihg, Cambridge,MA, USA, 2003. GARDNER, D.K., WEISSMAN, A., HOWLES, C.M., SHOHAM Z.,Textbook of Assisted Reproductive Techniques. Laboratory ant Clinical Perspectives. 2nd edition. Taylor & Francis, London, UK, 2004. HAFEZ, E.S.E., HAFEZ, B., Reproduction in farm animals. Lippincott Williams&Wilkins, 2000. LINDSAY, D., Breeding the flock, Inkata press, 1998.
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	0	0
Final Exam	1	100
Total	1	100
Contribution of Term (Year) Learning Activities to Success Grade		0
Contribution of Final Exam to Success Grade		100
Total		100
Measurement and Evaluation Techniques Used in the Course
Information

ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	1	14
Practicals/Labs	14	2	28
Self Study and Preparation	10	5	50
Homeworks, Performances	0	5	10
Projects	1	6	6
Field Studies	0	0	0
Midtermexams	0	0	0
Others	0	0	0
Final Exams	1	42	42
Total WorkLoad			150
Total workload/ 30 hr			5
ECTS Credit of the Course			5

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

	PQ2	PQ3	PQ4	PQ5	PQ6	PQ7	PQ8	PQ9	PQ10	PQ11	PQ12
LO1	0	5	3	0	0	3	3	3	4	4	5
LO2	3	5	4	0	0	3	3	3	3	4	5
LO3	0	0	0	2	5	2	0	0	0	0	0
LO4	0	0	0	2	5	2	0	3	0	0	0
LO5	4	4	1	0	0	5	2	2	2	2	4
LO6	0	5	3	0	0	4	5	5	5	4	5
LO7	3	4	0	0	0	3	4	4	4	4	4
LO8	0	2	1	0	5	0	3	3	3	0	0

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High