1	Course Title:	MOLECULAR BIOLOGY TECHNIQUES IN HORTICULTURE
2	Course Code:	BAB5025
3	Type of Course:	Optional
4	Level of Course:	Second Cycle
5	Year of Study:	1
6	Semester:	1
7	ECTS Credits Allocated:	6
8	Theoretical (hour/week):	2
9	Practice (hour/week) :	2
10	Laboratory (hour/week) :	0
11	Prerequisites:	-
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. HATİCE GÜLEN
16	Course Lecturers:	Prof.Dr. Meryem İpek Doç.Dr. Ahmet İpek
17	Contactinformation of the Course Coordinator:	hsgulen@uludag.edu.tr 224-2941477 Uludağ Üniversitesi Ziraat Fakültesi Bahçe Bitkileri Bölümü Nilüfer/Bursa
18	Website:
19	Objective of the Course:	The purpose of this course is to advanced information about molecular biology techniques and their use in Horticulture in theory and laboratory practices.
20	Contribution of the Course to Professional Development

21

Learning Outcomes:

1 Learn molecular biology topics in advanced level; 2 Learn the techniques in molecular biology; 3 Learn the laboratory analysis used in molecular biology. ; 4 Learn the applications of molecular biology in horticulture; 5 Learn molecular biology techniques in plant breeding; 6 Learn the application of molecular biology techniques in growing horticultural crops; 7 Develop innovative solutions using molecular biology techniques in horticulture;

22	Course Content:

Week	Theoretical	Practical
1	Giving outlines and the textbooks of the course.	Giving outlines of the practice
2	Structure of enzymes and proteins	Enzyme extraction, determination of enzyme activity
3	Enzyme markers	Native-PAGE
4	Protein markers-I	Protein extraction and protein content
5	Protein markers-II	SDS-PAGE
6	Structure of DNA and RNA-I	DNA extraction
7	Structure of DNA and RNA-II	RNA extraction-I
8	Structure of genes-I	RNA extraction-II
9	Structure of genes-II	DNA concentration
10	PCR techniques-I	RAPD
11	PCR techniques-II	SSR-I
12	PCR techniques-III	SSR-II
13	PCR techniques-IV	Data analysis-I
14	PCR techniques-V	Data analysis-II

23	Textbooks, References and/or Other Materials:	Acquaah, G. 1992. Practical Protein Electrophoresis for Genetic Research. Dioscorides Press, Theodore R.Dudley, PhD., General Editor, Portland, Oregon, 131 p. Alberts, B., Bray, D., Lewis, J., Raff, M., Roberts, K. And Watson, J.D. 1994. Molecular Biology of The Cell. Garland Publishing, Inc., New York & London, 1294 p. Eriş, A. Ve Gülen, H. Moleküler Biyoloji (Temel Bilgiler). Uludağ Üniv. Ziraat Fak. Ders Notları No: , 136 s. Özcan, S., Gürel, E. Ve babaoğlu, M. 2001. Bitki Biyoteknolojisi-II (Genetik Mühendisliği ve Uygulamaları). Selçuk Üniv. Vakfı. Yay., Konya, 456 s. Temizkan, G. Ve Arda, N. 1999. Moleküler Biyolojide Kullanılan Yöntemler. Nobel Tıp Kitabevi Ltd, 236 s.
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	3	50
Final Exam	1	50
Total	4	100
Contribution of Term (Year) Learning Activities to Success Grade		50
Contribution of Final Exam to Success Grade		50
Total		100
Measurement and Evaluation Techniques Used in the Course
Information

25

ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	2	28
Practicals/Labs	14	2	28
Self Study and Preparation	14	1	14
Homeworks, Performances	3	12	36
Projects	0	0	0
Field Studies	0	0	0
Midtermexams	0	0	0
Others	0	0	0
Final Exams	1	72	72
Total WorkLoad			178
Total workload/ 30 hr			5,93
ECTS Credit of the Course			6

26	CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
PQ1 PQ2 PQ3 PQ4 PQ5 PQ6 PQ7 PQ8 PQ9 PQ10 LO1 0 4 0 5 0 5 3 2 0 4 LO2 0 4 0 5 0 5 3 2 0 4 LO3 0 4 0 5 0 5 3 2 0 4 LO4 0 4 0 5 0 5 3 2 0 4 LO5 0 4 0 5 0 5 3 2 0 4 LO6 0 4 0 5 0 5 3 2 0 4 LO7 0 4 0 5 0 5 3 2 0 4

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High