1	Course Title:	FREE FLOATING SECTION TECHNIQUE AND IMMUNOFLOURESANS METHOD
2	Course Code:	VHE6019
3	Type of Course:	Optional
4	Level of Course:	Third Cycle
5	Year of Study:	1
6	Semester:	1
7	ECTS Credits Allocated:	3
8	Theoretical (hour/week):	2
9	Practice (hour/week) :	0
10	Laboratory (hour/week) :	0
11	Prerequisites:
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Dr. Ögr. Üyesi Tuncay İLHAN
16	Course Lecturers:
17	Contactinformation of the Course Coordinator:	Tel: 0224 2941266 E-mail: tilhan@uludag.edu.tr Bursa Uludağ Üniversitesi Veteriner Fakültesi A Blok Histoloji-Embriyoloji A.D.
18	Website:
19	Objective of the Course:	To teach free-floating section technique and immunofluorescent staining used in histology laboratory.
20	Contribution of the Course to Professional Development	It will be competent to use these techniques in scientific laboratory studies in the field of histology.

21

Learning Outcomes:

1 Ability to use total perfusion technique.; 2 Learning the usage areas and technical details of free floating section technique.; 3 Learning the application of immunofluorescence staining technique on free floating sections.; 4 To learn using cryotome in histology laboratory.;

22	Course Content:

Week	Theoretical	Practical
1	Routine histology technique.
2	Use of a microtome.
3	Tissue detection, fixing solutions, solutions appropriate to cryo-section.
4	Total perfusion technique.
5	Usage areas of cryotome, reasons of preference.
6	Using cryotome, collecting free floating sections.
7	Preparation of Cryo protector solution and washing solutions.
8	Washing of tissues, selection of appropriate sections.
9	Selection of appropriate primary antibody and fluorescent labeled secondary antibodies.
10	Application of fluorescent staining 1 (primary antibody in well plate)
11	Application of fluorescent staining 2 (Use of fluorescently labeled antibodies)
12	Taking free floating sections on to microscope slides, use of fluorescence microscope, storage of sections.
13	Double immunofluorescent staining.
14	Free floating section technique in central nervous system.

23	Textbooks, References and/or Other Materials:	Immunohistochemistry: Basics and Methods. Igor B. BuchwalowWerner Böcker.
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	0	0
Final Exam	1	100
Total	1	100
Contribution of Term (Year) Learning Activities to Success Grade		0
Contribution of Final Exam to Success Grade		100
Total		100
Measurement and Evaluation Techniques Used in the Course	The evaluation will be done with open-ended questions, short answer questions and multiple choice questions.
Information

25

ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	2	28
Practicals/Labs	0	0	0
Self Study and Preparation	14	4	56
Homeworks, Performances	0	0	0
Projects	0	0	0
Field Studies	0	0	0
Midtermexams	0	0	0
Others	0	0	0
Final Exams	1	1	1
Total WorkLoad			85
Total workload/ 30 hr			2,83
ECTS Credit of the Course			3

26	CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
PQ1 PQ2 PQ3 PQ4 PQ5 PQ6 PQ7 PQ8 PQ9 PQ10 PQ11 LO1 3 4 5 4 5 5 4 4 5 5 5 LO2 4 4 4 5 4 5 5 5 5 4 5 LO3 5 5 4 5 4 3 4 4 5 4 4 LO4 5 4 5 5 4 5 5 5 4 4 5

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High