1	Course Title:	WESTERN BLOT METHOD
2	Course Code:	VHE6018
3	Type of Course:	Optional
4	Level of Course:	Third Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	3
8	Theoretical (hour/week):	2
9	Practice (hour/week) :	0
10	Laboratory (hour/week) :	0
11	Prerequisites:	None
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Dr. Ögr. Üyesi SABİRE GÜLER
16	Course Lecturers:	-
17	Contactinformation of the Course Coordinator:	sabirepr@uludag.edu.tr 02242941261 Uludağ Ünv. Veteriner Fak. Histoloji Embriyoloji Anabilim Dalı
18	Website:	http://www.veteriner.uludag.edu.tr
19	Objective of the Course:	To determine the specific protein in cells or tissue.
20	Contribution of the Course to Professional Development	By determining the desired protein in cells, tissues and organs; It is to contribute to the diagnosis of various disease symptoms and to understand an important diagnostic method for molecular studies.

21

Learning Outcomes:

1 Learn the purpose and principles of the method.; 2 Prepare the tissue homogenate or extract.; 3 Learn the amount of total protein in the tissues ; 4 Learn preparation an agarose gel ; 5 Buffer solutions preparation and learns the principles. ; 6 Make specific protein analysis of tissue or cell; 7 Learn Western blot imaging method; 8 Learn use fields; 9 Evaluate the results.; 10 Make the solutions to problems that arise.;

22	Course Content:

Week	Theoretical	Practical
1	The basic principle of the method of electrophoresis.
2	Application areas
3	Introducing of gels
4	Usage of SDS gel electrophoresis
5	Principal of SDS –PAGE electrophoresis
6	Factors affecting the electrophoretic migration of proteins.
7	Acrilamid concentration
8	Buffer solutions used in the SDS-PAGE
9	Membrans and differences between membrans
10	Western blot protocol
11	Labeling and staining of specific proteins
12	Western blot imaging tecniques
13	Analysing western blot bands
14	Troubleshooting

23	Textbooks, References and/or Other Materials:	1. S Yılmazer, M Öztürk , Ş Arı. Moleküler Biyolojide Kullanılan Yöntemler, Ed. Güler Temizkan, Nazlı Arda. Nobel Tıp Kitapevi, İstanbul, 1999 2. Ed. Hames B.D. Gel Elektrophoresis of Protein. Oxford University Press, USA; 3 edition (December 10, 1998) ISBN-13: 978-0199636402 3. Methods in Molecular Biology Series Ed.: Walker, J.M. ISSN: 1064-3745 June 2009
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	0	0
Final Exam	1	100
Total	1	100
Contribution of Term (Year) Learning Activities to Success Grade		0
Contribution of Final Exam to Success Grade		100
Total		100
Measurement and Evaluation Techniques Used in the Course	Applied evaluation will be made with open-ended questions.
Information

25

ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	2	28
Practicals/Labs	0	0	0
Self Study and Preparation	14	2	28
Homeworks, Performances	0	28	28
Projects	0	0	0
Field Studies	0	0	0
Midtermexams	0	0	0
Others	0	0	0
Final Exams	1	2	2
Total WorkLoad			86
Total workload/ 30 hr			2,87
ECTS Credit of the Course			3

26	CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
PQ1 PQ2 PQ3 PQ4 PQ5 PQ6 PQ7 PQ8 PQ9 PQ10 PQ11 LO1 5 5 5 5 5 5 4 4 3 3 5 LO2 5 5 5 5 5 5 4 4 3 3 5 LO3 5 5 5 5 5 5 4 4 3 3 5 LO4 5 5 5 5 5 5 4 4 3 3 5 LO5 5 5 5 5 5 5 4 4 3 3 5 LO6 5 5 5 5 5 5 4 4 3 3 5 LO7 5 5 5 5 5 5 4 4 3 3 5 LO8 5 5 5 5 5 5 4 4 3 3 5 LO9 5 5 5 5 5 5 4 4 3 3 5 LO10 5 5 5 5 5 5 4 4 3 3 5

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High