1	Course Title:	IMMUNOHISTOCHEMISTRY OF REPRODUCTIVE BIOLOGY
2	Course Code:	TÜB5016
3	Type of Course:	Optional
4	Level of Course:	Second Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	3
8	Theoretical (hour/week):	1
9	Practice (hour/week) :	2
10	Laboratory (hour/week) :	0
11	Prerequisites:	NONE
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. ZEHRA MİNBAY
16	Course Lecturers:	Prof. Dr. Zehra MİNBAY Prof. Dr. Özhan EYİGÖR Dr. Öğr. Üyesi Duygu GÖK YURTSEVEN
17	Contactinformation of the Course Coordinator:	zminbay@uludag.edu.tr (224) 295 40 64 Uludağ Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, 16059 Nilüfer, BURSA
18	Website:
19	Objective of the Course:	The aim of this course is to explain basic principles and usage areas and to enable to use of immunohistochemical techniques
20	Contribution of the Course to Professional Development	This course is important in terms of providing basic knowledge and skills within the scope of in master degree education.

21

Learning Outcomes:

1 Realize all steps of the immunohistochemical labeling procedures.; 2 Choose the appropriate antibodies to be used in immunohistochemical labeling and prepare the necessary solutions for immunohistochemical methods.; 3 Carry out all steps of immunohistochemical techniques.; 4 Select immunohistochemical method used on an experimental research. ; 5 Evaluate the results of light microscope and makes quantitative analysis;

22	Course Content:

Week	Theoretical	Practical
1	Methods for proteins detection: similarities and differences What is immunohistochemistry? Fields of application, advantages and disadvantages	Overview of the immunohistochemistry laboratory
2	Immunoglobulins: Types, structure and function	Overview of the immunohistochemistry laboratory equipment
3	Tissue preparation for immunohistochemistry - I – Fixation and fiksatives	Fixation (perfusion and immersion fixation)
4	Tissue preparation for immunohistochemistry II – Tissue processing and sectioning	Sectioning with microtome
5	Tissue preparation for immunohistochemistry III – Pre-treatments in immunohistochemistry	Sectioning with cryostat
6	Antibodies and reagents in immunohistochemistry I – Antibody diluents, washing buffer and blocking buffer	Antigen retrieval
7	Antibodies and reagents in immunohistochemistry II – Primary antibodies, choice and optimization of primer antibody	Preparation of washing buffer and blocking buffer and cryoprotectant for free-floating section
8	Antibodies and reagents in immunohistochemistry III – secondary antibodies, properties and choice of sek. antibody	Sectioning with vibrotome
9	Detection methods I – Chromogenic techniques, enzyme conjugates and chromogenic substrates	Immunohistochemistry for parafin-embedded section
10	Detection methods II – Chromogenic techniques, enzyme conjugates and chromogenic substrates	Immunohistochemistry for free-floating sections
11	Detection methods III - Fluorochromes and immunofluorescences techniques	Double immunoflouresans staining method
12	IHC controls	Light microscopical analysis of the preparations
13	Problems in immunohistochemistry and troubleshooting	Fluorescence microscopical analysis of the preparations
14	Analysis of samples and principles of quantitative analysis of immunohistochemical staining	Quantitative analysis of immunohistochemical stainings

23	Textbooks, References and/or Other Materials:	1. Kalyuzhny AE. Immunohistochemistry. Essential Elements and Beyond. Switzerland: Springer International Publishing; 2016. 2. Renshaw S.Immunohistochemistry and Immunocytochemistry_ Essential Methods. 2nd edition. West Sussex:Wiley-Blackwell; 2017. 3. Lin F, Prichard J. Handbook of Practical Immunohistochemistry_ Frequently Asked Questions. 2nd edition. New York: Springer-Verlag; 2015. 4. Buchwalow IB, Böcker W. Immunohistochemistry_ Basics and Methods. Berlin Heidelberg: Springer-Verlag; 2010. 5. Burns R. Immunohistochemical Protocols. 3rd edition. Totowa: Humana Press; 2005. 6. Kumar GL. Education Guide – Immunohistochemical (IHC) Staining Methods. 5th edition. California: DAKO, 2009.
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	0	0
Final Exam	1	100
Total	1	100
Contribution of Term (Year) Learning Activities to Success Grade		0
Contribution of Final Exam to Success Grade		100
Total		100
Measurement and Evaluation Techniques Used in the Course	Measurement and evaluation are performed according to the Rules & Regulations of Bursa Uludağ University on Undergraduate Education.
Information

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ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	1	14
Practicals/Labs	14	2	28
Self Study and Preparation	14	2	28
Homeworks, Performances	0	0	0
Projects	0	0	0
Field Studies	0	0	0
Midtermexams	0	0	0
Others	3	4	12
Final Exams	1	10	10
Total WorkLoad			92
Total workload/ 30 hr			3,07
ECTS Credit of the Course			3

26	CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
PQ1 PQ2 PQ3 PQ4 PQ5 PQ6 PQ7 PQ8 PQ9 PQ10 PQ11 LO1 3 0 0 5 3 3 0 0 2 0 3 LO2 3 0 0 5 3 3 0 0 2 0 3 LO3 3 0 0 5 3 3 0 0 2 0 3 LO4 3 0 0 5 3 3 0 0 2 0 3 LO5 3 0 0 5 3 3 0 0 2 0 3

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High