COURSE SYLLABUS
FREE FLOATING SECTION TECHNIQUE AND IMMUNOFLOURESANS METHOD
| 1 |
Course Title: |
FREE FLOATING SECTION TECHNIQUE AND IMMUNOFLOURESANS METHOD |
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Course Code: |
VHE6023 |
| 3 |
Type of Course: |
Optional |
| 4 |
Level of Course: |
Third Cycle |
| 5 |
Year of Study: |
1 |
| 6 |
Semester: |
1 |
| 7 |
ECTS Credits Allocated: |
3 |
| 8 |
Theoretical (hour/week): |
2 |
| 9 |
Practice (hour/week) : |
0 |
| 10 |
Laboratory (hour/week) : |
0 |
| 11 |
Prerequisites: |
|
| 12 |
Recommended optional programme components: |
None |
| 13 |
Language: |
Turkish |
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Mode of Delivery: |
Face to face |
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Course Coordinator: |
Dr. Ögr. Üyesi Tuncay İLHAN |
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Course Lecturers: |
|
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Contactinformation of the Course Coordinator: |
Tel: 0224 2941266
E-mail: tilhan@uludag.edu.tr
Bursa Uludağ Üniversitesi Veteriner Fakültesi A Blok Histoloji-Embriyoloji A.D. |
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Website: |
|
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Objective of the Course: |
To teach free-floating section technique and immunofluorescent staining used in histology laboratory. |
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Contribution of the Course to Professional Development |
|
| 21 |
Learning Outcomes: |
| 1 |
Ability to use total perfusion technique.;
|
| 2 |
Learning the usage areas and technical details of free floating section technique.;
|
| 3 |
Learning the application of immunofluorescence staining technique on free floating sections.;
|
| 4 |
To learn using cryotome in histology laboratory.;
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|
| Week |
Theoretical |
Practical |
| 1 |
Routine histology technique. |
|
| 2 |
Use of a microtome. |
|
| 3 |
Tissue detection, fixing solutions, solutions appropriate to cryo-section. |
|
| 4 |
Total perfusion technique. |
|
| 5 |
Usage areas of cryotome, reasons of preference. |
|
| 6 |
Using cryotome, collecting free floating sections. |
|
| 7 |
Preparation of Cryo protector solution and washing solutions. |
|
| 8 |
Washing of tissues, selection of appropriate sections. |
|
| 9 |
Selection of appropriate primary antibody and fluorescent labeled secondary antibodies. |
|
| 10 |
Application of fluorescent staining 1 (primary antibody in well plate) |
|
| 11 |
Application of fluorescent staining 2 (Use of fluorescently labeled antibodies) |
|
| 12 |
Taking free floating sections on to microscope slides, use of fluorescence microscope, storage of sections. |
|
| 13 |
Double immunofluorescent staining. |
|
| 14 |
Free floating section technique in central nervous system. |
|
| 23 |
Textbooks, References and/or Other Materials: |
Immunohistochemistry: Basics and Methods. Igor B. BuchwalowWerner Böcker. |
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Assesment |
|
| TERM LEARNING ACTIVITIES |
NUMBER |
PERCENT |
| Midterm Exam |
0 |
0 |
| Quiz |
0 |
0 |
| Homeworks, Performances |
0 |
0 |
| Final Exam |
1 |
100 |
| Total |
1 |
100 |
| Contribution of Term (Year) Learning Activities to Success Grade |
0 |
| Contribution of Final Exam to Success Grade |
100 |
| Total |
100 |
| Measurement and Evaluation Techniques Used in the Course |
|
| Information |
|
| 25 |
|
ECTS / WORK LOAD TABLE |
| Activites |
NUMBER |
TIME [Hour] |
Total WorkLoad [Hour] |
| Theoretical |
14 |
2 |
28 |
| Practicals/Labs |
0 |
0 |
0 |
| Self Study and Preparation |
14 |
4 |
56 |
| Homeworks, Performances |
0 |
0 |
0 |
| Projects |
0 |
0 |
0 |
| Field Studies |
0 |
0 |
0 |
| Midtermexams |
0 |
0 |
0 |
| Others |
0 |
0 |
0 |
| Final Exams |
1 |
1 |
1 |
| Total WorkLoad |
|
|
85 |
| Total workload/ 30 hr |
|
|
2,83 |
| ECTS Credit of the Course |
|
|
3 |
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CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS |
|
|
| LO: Learning Objectives |
PQ: Program Qualifications |
| Contribution Level: |
1 Very Low |
2 Low |
3 Medium |
4 High |
5 Very High |
