COURSE SYLLABUS

TECHNIQUES OF ELECTRON MICROSCOPY

1	Course Title:	TECHNIQUES OF ELECTRON MICROSCOPY
2	Course Code:	THE 6002
3	Type of Course:	Compulsory
4	Level of Course:	Third Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	5
8	Theoretical (hour/week):	1
9	Practice (hour/week) :	4
10	Laboratory (hour/week) :	0
11	Prerequisites:	THE 6005
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. İLKİN ÇAVUŞOĞLU
16	Course Lecturers:	Doç.Dr. Berrin Avcı
17	Contactinformation of the Course Coordinator:	ilkin@uludag.edu.tr +90 224 2954051 Uludağ University Faculty of Medicine Histology and Embryology Department 16059 BURSA / TURKEY
18	Website:
19	Objective of the Course:	Edification of research specialists who can; get to know and use electron microscope, plan and carry out electron microscopical investigations, use electron microscopical techniques accurately and perfectly, solve technical problems and develop new methods.
20	Contribution of the Course to Professional Development

Learning Outcomes:

1	Can compare the structural properties of electron microscope with light microscope;
2	Can explain the operational principle of the electron microscope;
3	Can describe the area of usage of the electron microscope;
4	Can implement routine electron microscopical processes in order and accurately;
5	Can correlate the cause and effect of electron microscopical processes;
6	Can practice different electron microscopical procedures;
7	Can recognize the equipments of electron microscopy laboratory;
8	Can plan and carry out electron microscopical investigations;
9	Can propose solutions for problems experienced in the electron microscopy laboratory;

22	Course Content:

Week	Theoretical	Practical
1	The aim and learning objectives of the course Short history of the electron microscope	Introduction of the electron microscopy laboratory
2	Structure of the electron microscope and the principles of function	Introduction of the electron microscope
3	Fixation and fixatives	Preparation of fixatives
4	Buffer solutions	Preparation of buffers
5	Principles of tissue preparation	Application of tissue preparation protocols
6	Embedding mediums for electron microscopy	Preparation of embedding mediums
7	Embeding procedures	Application of tissue embedding methods
8	Principles of sectioning	Preparing glass knifes
9	Features of thick and thin sections	Sectioning by using ultratome
10	Methods and materials for staining thin sections	Staining of thin sections
11	Principles of examination, evaluation and photographing on TEM	Practice of examination and photographing on TEM
12	Principles and methods of the dark room	Practice of developing and printing micrographs
13	Evaluation methods for electron micrographs	Practice of evaluation of electron micrographs
14	Electron microscopical artifacts and problem solving	Precaution and removing artifacts

23	Textbooks, References and/or Other Materials:	1. Principles and Techniques of Electron Microscopy Biological Applications, 4th Edition. M.A. HAYAT. Cambridge University Press, 2000. 2. Biological Electron Microscopy Theory, Techniques and Troubleshooting, 2nd Edition. M.J. DYKSTRA and L.E. REUSS. Kluwer Academic/Plenum Publishers, 2003.
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	0	0
Final Exam	1	100
Total	1	100
Contribution of Term (Year) Learning Activities to Success Grade		0
Contribution of Final Exam to Success Grade		100
Total		100
Measurement and Evaluation Techniques Used in the Course
Information

ECTS / WORK LOAD TABLE

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High