COURSE SYLLABUS

PLANT TISSUE CULTURE TECHNIQUES

1	Course Title:	PLANT TISSUE CULTURE TECHNIQUES
2	Course Code:	TAR3303-Z
3	Type of Course:	Compulsory
4	Level of Course:	First Cycle
5	Year of Study:	3
6	Semester:	5
7	ECTS Credits Allocated:	4
8	Theoretical (hour/week):	2
9	Practice (hour/week) :	2
10	Laboratory (hour/week) :	0
11	Prerequisites:	----
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. NAZAN DAĞÜSTÜ
16	Course Lecturers:	---
17	Contactinformation of the Course Coordinator:	ndagustu@uludag.edu.tr, 224 2941518, U.U. Field Crops Department, Faculty of Agriculture 16059 Görükle Campus Bursa
18	Website:
19	Objective of the Course:	The objectives of courses are; to follow the new technologies and applications on agricultural biotechnology, get enough information on biotechnolgy, get experience on applications of agricultural biotechnology
20	Contribution of the Course to Professional Development	It will be realized that the students who will work in the plant tissue culture laboratory have knowledge and experience on the subject.

Learning Outcomes:

1	No sterilization problems in the plant tissue culture laboratory ;
2	Have the knowledge of how to plan tissue culture laboratory ;
3	Have technical knowledge and education degree in tissue culture ;
4	Gains experience in preparing the fattening medium ;
5	Have knowledge about plant tissue culture methods ;
6	Knows how to choose the right method in plant tissue culture techniques applications;
7	Learns the rules and practices that need to be considered while working in the laboratory ;
8	Gains experience in putting this new technique into practice in the current field.;
9	It can work without any difficulty in a commercial plant tissue culture laboratory. ;
10	Can work on any research project ;

22	Course Content:

Week	Theoretical	Practical
1	The points to be considered in the laboratory studies, the introduction of the departments where the plant tissue culture laboratory studies are carried out, the introduction of the tools, equipment and materials used in the laboratory	Issues to be considered in laboratory studies
2	Chemicals used in the laboratory, Stock solutions, Preparation of the nutrient medium	Laboratory accidents
3	Sterilization	Demonstration of the instruments and equipment of the departments in the tissue culture laboratory
4	Isolation, inoculation and incubation of plant material	Demonstration of chemicals used in the laboratory
5	Somaklonal varyasyon	Preparation of stock solution
6	Somatic embryogenesis	Solution preparation in various concentrations
7	Organogenesis	Preparing the culture medium
8	Callus Culture, Cell suspension culture	Student presentation
9	Midterm exam	Midterm exam
10	Embriyo culture	Student presentation
11	Haploid Breeding- Anther culture and microspore culture	Student presentation
12	Meristem Culture	Student presentation
13	In vitro conservation of gene resources	Student presentation
14	Seconder metabolite prodution	Student presentation

23	Textbooks, References and/or Other Materials:	1. Bitki Biyoteknolojisi I Doku Kültürü ve Uygulamaları, 2001. M. Babaoğlu, E. Gürel, S. Özcan. S. U. Vakfı Yayınları, Konya. pp.1-456. 2. Bitki Biyoteknolojisi, 1999. R. Hatipoğlu, Ç.Ü. Ziraat Fakültesi Genel Yayın No: 190 Ders Kitapları Yayın No:A-58. 3. Plant Propagation by Tissue Culture. Handbook and Directory of Commercial Laboratories, 1984. Eds. E.F. George and P.D. Sherrington, Exegetics Ltd., England. 4. Handbook of Plant Cell Culture, Volume 1. Techniques for Propagation and Breeding, 1983. Eds. D.E. Evans, W.R. Sharp, P.V. Ammirato, Y. Yamada Macmillan Publishing Co. New York, pp. 1-970. 5. Experiments in Plant Tissue Culture, 1982. J.H. Dodds and L.W. Roberts, Cambridge University Press, UK. 6. Cell Culture and Somatic Cell Genetics of Plants, Volume 1 Laboratory Procedures and Their Applications, 1984. Ed. I.K. Vasil. Academic Press, Inc. New York, pp. 1-825. 7. Plant Cell Culture A Practical Approach, 1985. Ed. R.A. Dixon, IRL Press Limited, England, pp. 1-236. 8. Plant Cell and Tissue Culture A laboratory Manual, 1982. Eds. J.Reinert, M.M. Yeoman, Springer-Verlag, pp. 1-83.
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	1	20
Quiz	0	0
Homeworks, Performances	1	20
Final Exam	1	60
Total	3	100
Contribution of Term (Year) Learning Activities to Success Grade		40
Contribution of Final Exam to Success Grade		60
Total		100
Measurement and Evaluation Techniques Used in the Course	Bursa Uludağ University is evaluated according to the principles of the Associate and Undergraduate Education Regulation
Information	Results are determined by the letter grade determined by the student automation system

ECTS / WORK LOAD TABLE

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High