COURSE SYLLABUS

DIAGNOSTIC METHODS IN MICROBIOLOGY-I

1	Course Title:	DIAGNOSTIC METHODS IN MICROBIOLOGY-I
2	Course Code:	TMK5002
3	Type of Course:	Compulsory
4	Level of Course:	Second Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	5
8	Theoretical (hour/week):	1
9	Practice (hour/week) :	4
10	Laboratory (hour/week) :	0
11	Prerequisites:	To be successful in Diagnostic Methods in Microbiology I
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. Cüneyt ÖZAKIN
16	Course Lecturers:
17	Contactinformation of the Course Coordinator:	ozakin@uludag.edu.tr, 0224.2954115, Uludağ Üniversitesi tıp Fakültesi Temel Tıp Bilimleri Binası, Tıbbi Mikrobiyoloji Anabilim Dalı, 16059 Nilüfer, Bursa
18	Website:
19	Objective of the Course:	To learn about fast, automated identification and sensitivity test methods, systems, molecular diagnosis and epidemiological analysis techniques in microbiology and to make applications specific to their areas of use.
20	Contribution of the Course to Professional Development	Integrating theoretical knowledge with clinical laboratory practice.

Learning Outcomes:

1	To know the methods used for diagnosis in microbiology,;
2	To be able to select and apply the appropriate diagnostic method for microbiological diagnosis;
3	To interpret the results obtained from the diagnostic methods;
4	To be able to compare microbiological diagnostic methods;

22	Course Content:

Week	Theoretical	Practical
1	Direct Diagnosis in Microbiology- Microscopic Diagnosis; Light Microscopy and Electron Microscopy	Bacteriology: Plaque reading and simple definitions
2	Direct Diagnosis in Microbiology- Culture; Gram positive cocci and their identification	Bacteriology: Panel evaluation, biochemical properties
3	Direct Diagnosis in Microbiology- Culture; Gram negative bacilli and their definitions	Bacteriology: Maldi TOF MS
4	Direct Diagnosis in Microbiology- Culture; Gram positive bacilli and their definitions	Tüberküloz: Mikroskopi; Flouresan ve EZN boyama yöntemi
5	Direct Diagnosis in Microbiology- Culture; Gram positive bacilli and their definitions	Mikoloji: Mikroskopi, makroskopi, germ tüp ve şeker asimilasyon testleri
6	Direct Diagnosis in Microbiology- Nucleic Amplification	Parasitology: Microscopy, macroscopy and Trichrome staining
7	Direct Diagnosis in Microbiology- Hybridization	ELISA: Macro ELISA method
8	Maldi TOF MS method	ELISA: Micro ELISA and ELFA technique
9	Flow Cytometry Methods	Serology: Aglutination, indirect hemagglutination
10	Indirect Diagnosis in Microbiology- Antigen and Antibody	Serology: Complement fixation test, Nefolometric method
11	Indirect Diagnosis in Microbiology- Precipitation, Agglutination	Immunology: Flow cytometry
12	Indirect Diagnosis in Microbiology- Neutralization, Complement fixation test	Immunology: Autoantibodies
13	Indirect Diagnosis in Microbiology- Enzyme Immuno Assay (EIA, ELISA, ELFA)	PCR: Amplification
14	Indirect Diagnosis in Microbiology- Immunoflourescent methods	PCR: Real Time PCR

23	Textbooks, References and/or Other Materials:	1- Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH (Eds). Manual of Clinical Microbiology 8th Edition ASM Press Washington DC (2003) 2- Isenberg HD (Ed). Clinical Microbiology Procedures Handbook 2nd Edition. ASM Press Washington DC (2004) 3- Brooks GF, Carroll KC, Butel JS, Morse SA. Jawetz, Melnick & Adelberg’s Medical Microbiology 24th Edition McGraw-Hill Comp. USA, (2007)
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	0	0
Final Exam	1	100
Total	1	100
Contribution of Term (Year) Learning Activities to Success Grade		0
Contribution of Final Exam to Success Grade		100
Total		100
Measurement and Evaluation Techniques Used in the Course	Classical exam is done.
Information	One final exam are scheduled.

ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	1	14
Practicals/Labs	14	4	56
Self Study and Preparation	14	2	28
Homeworks, Performances	0	15	30
Projects	0	0	0
Field Studies	0	0	0
Midtermexams	0	0	0
Others	0	0	0
Final Exams	1	20	20
Total WorkLoad			148
Total workload/ 30 hr			4,93
ECTS Credit of the Course			5

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

	PQ1	PQ2	PQ3	PQ4	PQ5	PQ6	PQ8	PQ9
LO1	2	0	2	1	1	5	1	0
LO2	2	0	1	1	2	5	1	2
LO3	2	1	0	1	2	4	1	0
LO4	2	2	2	2	2	2	1	1

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High