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Course Title: |
REPRODUCTIVE BIOTECHNOLOGY |
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Course Code: |
VET4507 |
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Type of Course: |
Optional |
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Level of Course: |
First Cycle |
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Year of Study: |
4 |
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Semester: |
7 |
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ECTS Credits Allocated: |
3 |
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Theoretical (hour/week): |
1 |
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Practice (hour/week) : |
2 |
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Laboratory (hour/week) : |
0 |
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Prerequisites: |
None |
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Recommended optional programme components: |
None |
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Language: |
Turkish |
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Mode of Delivery: |
Face to face |
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Course Coordinator: |
Doç. Dr. BURCU ÜSTÜNER |
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Course Lecturers: |
Prof.Dr.İbrahim DOĞAN
Doç.Dr.Selim ALÇAY |
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Contactinformation of the Course Coordinator: |
e-posta: bbaspinar@uludag.edu.tr
Telefon: 0224-2941245
Adres: Bursa Uludağ Üniversitesi Veteriner Fakültesi Dölerme ve Suni Tohumlama Anabilim Dalı |
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Website: |
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Objective of the Course: |
Giving basic information about reproductive biotechnology and showing the possibilities of using reproductive biotechnology in Veterinary Medicine; determining the some practical application fields for improving animal husbandry in the country; providing enough knowledge and practice to the students in this field to apply some reproductive biotechnology techniques |
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Contribution of the Course to Professional Development |
The student transfer the knowledge gained in course to the field in professional practices and to support animal breeding methods with biotechnological applications. |
| Week |
Theoretical |
Practical |
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Description of in vitro embryo production (IVP), advantages of IVP, factors affecting the success of IVP. |
Presentation of IVF laboratory. |
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Methods used for oocyte collection from slaughterhouse material: aspiration, slicing, follicle dissection, media used for oocyte wash. |
Presentation of chemical agents and medium preparation. |
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Oocyte and embryo collection from living animals: oocyte and embryo collection by chirurgical method, oocyte collection using ultrasound guided transvaginal oocyte aspiration method (ovum pick-up, OPU). |
Oocyte collection. |
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Maturation of immature oocyte in laboratory conditions (in vitro maturation, IVM). |
IVM protocol. |
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Fertilization of in vitro matured oocytes in the laboratory (in vitro fertilization, IVF), most used methods for the preparation of sperm used for IVF: swim-up and percoll gradient system. |
IVM protocol. |
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Intracytoplasmic sperm injection (ICSI). |
IVF protocol. |
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Classifications of embryos according to the developmental stages: zygote, 2-cell, 4-cell, 8-cell, 16-cell, morula and blastocyst, morphological evaluation in embryos: excellent, fair, medium, poor. |
IVF protocol. |
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Culture environments for embryo survival after IVF: in vivo and in vitro cultures, media used for in vitro culture (IVC), co-culture, simplex (defined) and complex (non-defined) culture systems. |
In vitro culture of embryos. |
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Description of cryopreservation, advantages of cryopreservation and methods used for cryopreservation: slow freezing, fast freezing and vitrification. |
Evaluation of embryos morphologically. |
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Cryopreservation of oocytes, embryos and ovarian tissue. |
Conventional freezing procedure. |
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Embryo sexing before embryo transfer, methods and advantages of embryo sexing. |
Vitrification procedure. |
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What is transgenic? Advantages and methods of transgenic animal production. |
Oocyte freezing. |
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Description of embryonic stem cells, their advantages and application possibilities of them in veterinary and medical fields. |
Fixation and dying procedures. |
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What is cloning? Main application possibilities, reproductive and therapeutic cloning, methods used for cloning. |
Fixation and dying procedures. |
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Textbooks, References and/or Other Materials: |
1. Scientific farm animal production : an introduction to animal science / Robert E. Taylor, Thomas G. Field. – 8th ed. – Upper Saddle River, NJ : Pearson Prentice Hall, 2004.
2. Reproductive technologies in farm animals / Ian Gordon. – Wallinford, Oxon: CABI Publishing, 2004.
3. Basic biotechnology / edited by Colin Ratledge and Bjørn Kristiansen. – 2nd ed. – Cambridge, UK : Cambridge University Press, 2004.
4. Bearden, Henry Joe, 1926-. Applied animal reproduction / H.Joe Bearden, John W. Fuquay, Scott T. Willard. – 6th ed. – Upper Saddle River, New Jersey : Pearson / Prentice Hal , 2004.
5. Textbook of assisted reproductive techniques: laboratory and clinical perspectives / edited by David K. Gardner...et al.. – 2nd ed. – London : Taylor & Francis, 2004.
6.Gordon, Ian R. Reproductive technologies in farm animals / Ian Gordon. – Wallinford, Oxon : CABI Publishing , 2004.
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Assesment |
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