1	Course Title:	FLOW CYTOMETRY
2	Course Code:	TİM5008
3	Type of Course:	Optional
4	Level of Course:	Second Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	8
8	Theoretical (hour/week):	1
9	Practice (hour/week) :	4
10	Laboratory (hour/week) :	0
11	Prerequisites:	None
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. FERAH BUDAK
16	Course Lecturers:
17	Contactinformation of the Course Coordinator:	Uludağ Üniversitesi, Tıp Fakültesi, İmmünoloji Anabilim Dalı, 16059, Nilüfer, BURSA E-posta: fbudak@uludag.edu.tr Tel: 2954134
18	Website:
19	Objective of the Course:	This course is aimed to the student to comprehend the clinical and research applications of flow cytometry.
20	Contribution of the Course to Professional Development

21

Learning Outcomes:

1 To understand the mechanism and structure of the flow cytometry; 2 To understand the methods used in flow cytometry; 3 To learn the clinical applications of flow cytometry; 4 To learn the application of flow cytometry for research purposes;

22	Course Content:

Week	Theoretical	Practical
1	Basic principles of flow cytometry, history	Introduction to Flow cytometer
2	introduction of flourochromes, flourochrome staing techniques	Quality control applications
3	Current uses of flow cytometry	The combination of fluorescent and compensation
4	Immunophenotyping	Test protocol designing
5	Diagnosis of Immunodeficiency	Immune deficiency pane
6	Diagnosis of leukemia and lymphoma	Leukemia and Lymphoma panels
7	Proliferation and activation tests	CFSE proliferation test
8	Analysis apoptosis	Anneksin V/PI staining
9	Intracellular cytokine measurement	Intracellular cytokine measurement
10	Ekstracellular cytokine measurement	Cytokine bead assay
11	Phagocytosis, chemotaxis, oxidative burst measurements	Respiratory burst test
12	Stem cell analysis, absolute CD4 counting	Absolute CD4 counting
13	DNA analysis	DNA cycle analysis
14	Data evaluation softwares, future uses	Application of flow cytometry softwares

23	Textbooks, References and/or Other Materials:	1. Deniz G., Demirel G.Y. “Akan hücre ölçer” Yelken Ajans Reklamcılık, Yayıncılık ve Matbaacılık, 1. Baskı (2014) 2. Turgeon M.L., "Immunology & Serology in Laboratory Medicine", Elsevier (2014). 3. Howard M. Shaıro “Practical Flow Cytometry, Wıley and Sons Inc. Publication 4. Baskı (2003).
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	5	50
Final Exam	1	50
Total	6	100
Contribution of Term (Year) Learning Activities to Success Grade		50
Contribution of Final Exam to Success Grade		50
Total		100
Measurement and Evaluation Techniques Used in the Course
Information

25

ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	1	14
Practicals/Labs	14	4	56
Self Study and Preparation	14	10	140
Homeworks, Performances	5	2	10
Projects	0	0	0
Field Studies	0	0	0
Midtermexams	0	0	0
Others	0	0	0
Final Exams	1	15	15
Total WorkLoad			235
Total workload/ 30 hr			7,83
ECTS Credit of the Course			8

26	CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
PQ1 PQ2 PQ3 PQ4 PQ5 PQ6 PQ7 LO1 0 0 5 0 0 0 0 LO2 0 0 5 0 0 0 0 LO3 0 0 5 0 0 0 0 LO4 0 0 5 0 0 0 0

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High