Uludağ Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İmmünoloji Bilim Dalı, 16059, Nilüfer, BURSA E-posta: oralb@uludag.edu.tr Tel: 2954114
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Website:
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Objective of the Course:
The aim of this course is to provide the student knowledge and skills required for performing and evaluating molecular immunological methods.
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Contribution of the Course to Professional Development
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Learning Outcomes:
1
To gain knowledge and skills needed for performing molecular immunological laboratory tests, especially used in research ;
2
To gain knowledge and skills needed for the evaluation of molecular immunological laboratory tests, especially used in research;
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Course Content:
Week
Theoretical
Practical
1
DNA and RNA isolation methods
DNA and RNA isolation methods
2
Bacterial transformation
Bacterial transformation
3
Preparation of plazmid DNA from transformed bacteria (Miniprep)
Preparation of plazmid DNA from transformed bacteria (Miniprep)
4
Restriction deigestion and agarose gel electrophoresis
Restriction deigestion and agarose gel electrophoresis
5
cDNA preparation
cDNA preparation
6
Conventional PCR
Conventional PCR
7
RFLP
RFLP
8
RT-PCR
RT-PCR
9
Cytokine gene polymorphism (SSP-PCR)
Cytokine gene polymorphism (SSP-PCR)
10
Sequencing methods
Sequencing methods
11
Gene transfer into mammalian cells by lipofection
Gene transfer into mammalian cells by lipofection
12
Staining of the mammalian cells transfected with ß-galaktosidase plasmid (X-gal)
Staining of the mammalian cells transfected with ß-galaktosidase plasmid (X-gal)
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Pulse-field agarose gel elektrophoresis
Pulse-field agarose gel elektrophoresis
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Viral vector design
Viral vector design
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Textbooks, References and/or Other Materials:
1. Thompson L., "Measuring Immunity: Basic and clinical practice", Elsevier Academi,c Press (2005). 2. Buyru N., Dalay N., Özgüç M., Öztürk M., Sakızlı, M. (Çeviri editörleri) "Hücrenin Moleküler Biyolojisi", TÜBA Yayınları, 1. Basım (2008) 3. Green M., Sambrook J., "Molecular Cloning: A Laboratory Manual", Cold Springer Harbor Laboratory Press (2012)
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Assesment
TERM LEARNING ACTIVITIES
NUMBER
PERCENT
Midterm Exam
0
0
Quiz
0
0
Homeworks, Performances
5
50
Final Exam
1
50
Total
6
100
Contribution of Term (Year) Learning Activities to Success Grade
50
Contribution of Final Exam to Success Grade
50
Total
100
Measurement and Evaluation Techniques Used in the Course
Information
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ECTS / WORK LOAD TABLE
Activites
NUMBER
TIME [Hour]
Total WorkLoad [Hour]
Theoretical
14
1
14
Practicals/Labs
14
4
56
Self Study and Preparation
14
4
56
Homeworks, Performances
5
10
50
Projects
0
0
0
Field Studies
0
0
0
Midtermexams
0
0
0
Others
0
0
0
Final Exams
1
4
4
Total WorkLoad
180
Total workload/ 30 hr
6
ECTS Credit of the Course
6
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CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
