COURSE SYLLABUS

GENE THERAPY IN IMMUNOLOGY

1	Course Title:	GENE THERAPY IN IMMUNOLOGY
2	Course Code:	TMİ6010
3	Type of Course:	Optional
4	Level of Course:	Third Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	5
8	Theoretical (hour/week):	1
9	Practice (hour/week) :	4
10	Laboratory (hour/week) :	0
11	Prerequisites:	NONEYok
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. BARBAROS ORAL
16	Course Lecturers:	-
17	Contactinformation of the Course Coordinator:	Uludağ Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İmmünoloji Bilim Dalı, 16059, Nilüfer, BURSA E-mail: oralb@uludag.edu.tr Phone: 2954114
18	Website:
19	Objective of the Course:	The aim of this course is to provide knowledge to the student about different gene transfer techniques and usage of potential immune therapeutic genes.
20	Contribution of the Course to Professional Development

Learning Outcomes:

1	To gain knowledge about the basic principals of gene therapy and its associated risks and challenges;
2	To comprehend the mechanisms of gene transfer techniques;
3	To be aware of some diseases that might be treatable by targeting immune genes;

22	Course Content:

23	Textbooks, References and/or Other Materials:	1. Thompson L., "Measuring Immunity: Basic and clinical practice", Elsevier Academi,c Press (2005). 2. Buyru N., Dalay N., Özgüç M., Öztürk M., Sakızlı, M. (Çeviri editörleri) "Hücrenin Moleküler Biyolojisi", TÜBA Yayınları, 1. Basım (2008) 3. Green M., Sambrook J., "Molecular Cloning: A Laboratory Manual", Cold Springer Harbor Laboratory Press (2012)
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	5	50
Final Exam	1	50
Total	6	100
Contribution of Term (Year) Learning Activities to Success Grade		50
Contribution of Final Exam to Success Grade		50
Total		100
Measurement and Evaluation Techniques Used in the Course
Information

ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	1	14
Practicals/Labs	14	4	56
Self Study and Preparation	14	1	14
Homeworks, Performances	5	10	50
Projects	0	0	0
Field Studies	0	0	0
Midtermexams	0	0	0
Others	0	0	0
Final Exams	1	11	11
Total WorkLoad			145
Total workload/ 30 hr			4,83
ECTS Credit of the Course			5

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

	PQ3	PQ5
LO1	0	5
LO2	0	5
LO3	4	2

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High

Week	Theoretical	Practical
1	Potential therapeutic genes in Immunology	DNA database screening
2	Viral vectors	Primer and probe design
3	Non-viral vectors	Calculation of primer and probe concentrations and preparation of master mix
4	Amplification of target DNA by PCR	Amplification of target DNA by PCR
5	Ligation into cloning vector and bacterial transformation	Ligation into cloning vector and bacterial transformation
6	Selection of positive colonies and plasmid purification (miniprep)	Selection of positive colonies and plasmid purification (miniprep)
7	Restriction, agarose gel electrophoresis and purification of target DNA fragman from gel	Restriction, agarose gel electrophoresis and purification of target DNA fragman from gel
8	Cloning into expression vector	Cloning into expression vector
9	Selection of positive colonies and plasmid purification (maxiprep)	Selection of positive colonies and plasmid purification (maxiprep)
10	Gene transfer of reporter gene (ß-galaktosidase) into HeLa cells by chemical transfection	Gene transfer of reporter gene (ß-galaktosidase) into HeLa cells by chemical transfection
11	Staining of transfected cells (X-gal)	Staining of transfected cells (X-gal)
12	Gene transfer of reporter gene (ß-galaktosidase, CAT) into HeLa cells by lipofection	Gene transfer of reporter gene (ß-galaktosidase, CAT) into HeLa cells by lipofection
13	Gene transfer of reporter gene (ß-galaktosidase) into HeLa cells by adenovirus	Gene transfer of reporter gene (ß-galaktosidase) into HeLa cells by adenovirus
14	Current gene therapy studies in Immunology	Cell extract preparation and determination of gene expression by CAT ELISA Test