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Course Title: |
OXIDATIVE SYSTEM AND ANTIOXIDANTS |
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Course Code: |
TBK 6005 |
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Type of Course: |
Optional |
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Level of Course: |
Third Cycle |
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Year of Study: |
1 |
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Semester: |
1 |
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ECTS Credits Allocated: |
5 |
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Theoretical (hour/week): |
1 |
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Practice (hour/week) : |
2 |
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Laboratory (hour/week) : |
0 |
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Prerequisites: |
No |
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Recommended optional programme components: |
None |
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Language: |
Turkish |
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Mode of Delivery: |
Face to face |
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Course Coordinator: |
Prof. Dr. ZEHRA SERDAR |
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Course Lecturers: |
- |
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Contactinformation of the Course Coordinator: |
zserdar@uludag.edu.tr
2953914
Uludağ Üniversitesi Tıp Fakültesi, Temel Tıp Bilimleri Binası, Tıbbi Biyokimya Anabilim Dalı, 16059
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Website: |
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Objective of the Course: |
a) To teach the structures, physical and chemical characteristics, metabolites, endogen and exogen sources of the free radicals, and cellular damages such as lipid peroxidation, protein and carbohydrate oxidation and DNA damage and, their roles at the development of the diseases.
b) To teach the structures and functions of the intracellular antioxidants, membrane antioxidants and extracellular antioxidants and, their roles at the development of the diseases.
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Contribution of the Course to Professional Development |
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| Week |
Theoretical |
Practical |
| 1 |
Description of the free radicals |
Introduction of spectrophotometer |
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Metabolites and chemical structures |
Measurement of MDA at spectrophotometer |
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Reactivite and half-life |
Measurement of conjuge dien at spectrophotometer
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Endogen sources |
Introduction of HPLC
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Exogen sources |
Measurement of MDA at HPLC |
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Role of free radicals in cellular damage development |
Measurement of protein carbonyls at spectrophotometer |
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Measurement methods of the free radicals |
Measurement of carotenoids at spectrophotometer |
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Description of the antioxidants |
Measurement of vitamin E at spectrophotometer |
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Intracellular antioxidants |
Measurement of vitamin E at HPLC |
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Membrane antioxidants |
Measurement of catalase at spectrophotometer |
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Extracellular antioxidants |
Measurement of SOD at spectrophotometer |
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Non-classifiable antioxidants |
Measurement of GPX at spectrophotometer |
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Measurement methods of the antioxidants |
Measurement of PON at spectrophotometer |
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The role of free radicals and antioxidants in the pathogenesis of several diseases |
Measurement of arylesterase at spectrophotometer |
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Textbooks, References and/or Other Materials: |
1. Kılınç K, Kılınç A. Oksijen toksisitesinin aracı molekülleri olarak oksijen radikalleri. Hacettepe Tıp Dergisi 2002; 33(2): 110 – 118.
2. Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J: Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol 39: 44-84, 2007.
3. Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS, Lele RD: Free radicals and antioxidants in human health: current status and future prospects. J Assoc Physicians India 52: 794-804, 2004.
4. Laguerre M, Lecomte J, Villeneuve P. Evaluation of the ability of antioxidants to counteract lipid oxidation: Existing methods, new trends and challenges. Progress in Lipid Research. 2007; 46: 244–282.
5. Halliwell B, Gutteridge J M C Free radicals in biology and medicine, UK. Clarendon press; 1989
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Assesment |
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