COURSE SYLLABUS

MATERIAL SAMPLING, SECTIONING AND STAINING TECHNIQUES

1	Course Title:	MATERIAL SAMPLING, SECTIONING AND STAINING TECHNIQUES
2	Course Code:	VPT 6003
3	Type of Course:	Compulsory
4	Level of Course:	Third Cycle
5	Year of Study:	1
6	Semester:	1
7	ECTS Credits Allocated:	5
8	Theoretical (hour/week):	1
9	Practice (hour/week) :	2
10	Laboratory (hour/week) :	0
11	Prerequisites:	None
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. M.MÜFIT KAHRAMAN
16	Course Lecturers:	-
17	Contactinformation of the Course Coordinator:	gursels@uludag.edu.tr, 224 2941303, Uludağ Üniv. Veteriner Fak. Patoloji Anabilim Dalı Görükle Kampüsü 16059 BURSA
18	Website:	http://saglikbilimleri.uludag.edu.tr
19	Objective of the Course:	To teach students the fixation, decalcification, processing of tissues, preparation of sections and staining methods for histopathological examination
20	Contribution of the Course to Professional Development

Learning Outcomes:

1	The student learns fixative solutions and application techniques, fixation problems and delcalcification techniques ;
2	The student learns processing of tissues and embedding in paraffin ;
3	The student learns preparation of sections ;
4	The student learns routine hematoxylin and eosin staining method ;
5	The student learns special staining techniques ;

22	Course Content:

Week	Theoretical	Practical
1	Preparation of tissues (fixation)	Preparation and application of fixative solutions
2	Preparation of tissues (decalcification)	Preparation and application of decalcifying solutions
3	Processing of tissues (dehydration, clearing, paraffin embedding)	Dehydrating, clearing and embedding in paraffin of tissues
4	Preparation of sections	Preparation of sections
5	Routine staining procedures (Hematoxylin and Eosin methods)	Routine Hematoxylin and Eosin staining
6	Staining methods of connective tissue (collagen, keratin, mucin, muscle)	Connective tissue staining
7	Staining methods of cytoplasmic granules (mast cells, chromaffin)	Mast cells staining
8	Staining methods of hematologic and nuclear elements	Giemsa staining
9	Staining methods of fats and lipids	Fat staining
10	Staining methods of carbohydrates and mucoproteins (amyloid, glycogen)	Amyloid and glycogen staining
11	Staining methods of pigments and minerals (biluribin, calcium, urate crystal)	Calcium and urate crystals staining
12	Staining methods of pigments and minerals (cooper, hemosiderin, iron)	Iron staining
13	Staining methods of nerve cells and fibers (astrocytes, nerve fibers)	Nerve fibers staining
14	Staining methods of bacteria and fungi (acid fast organisms, Gram, fungi)	Bacteria and fungi staining

23	Textbooks, References and/or Other Materials:	Histological and Histochemical Technics (Davenport, H.A., 1960, W.B.Saunders Comp, Philadelphia) Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology (Luna, L.G., 1969, McGraw-Hill Book Comp, London) Theory and Practice of Histological Techniques (Bancroft, J.D., Gamble, M., 2002, Churchill Livingstone, London) Histological and Histochemical Methods: Theory and Practice (Kiernan, J.A., 2008, Scion Publishing Ltd, Oxfordshire)
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	0	0
Final Exam	1	100
Total	1	100
Contribution of Term (Year) Learning Activities to Success Grade		0
Contribution of Final Exam to Success Grade		100
Total		100
Measurement and Evaluation Techniques Used in the Course
Information

ECTS / WORK LOAD TABLE

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High