COURSE SYLLABUS

TECHNIQUES OF HISTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY

1	Course Title:	TECHNIQUES OF HISTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY
2	Course Code:	THE 6004
3	Type of Course:	Compulsory
4	Level of Course:	Third Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	5
8	Theoretical (hour/week):	1
9	Practice (hour/week) :	4
10	Laboratory (hour/week) :	0
11	Prerequisites:	THE 6005 Tecniques of Light Microscopy
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. ZEHRA MİNBAY
16	Course Lecturers:	Prof.Dr. Zehra Minbay
17	Contactinformation of the Course Coordinator:	oeyigor@uludag.edu.tr 295 4065 U.Ü. Tıp Fak. Histoloji ve Embriyoloji AD.
18	Website:
19	Objective of the Course:	To educate researchers; who comprehend the detailed knowledge of histochemical and immunohistochemical techniques, who acknowledge the area of usage and the differences of these techniques and who can employ these techniques.
20	Contribution of the Course to Professional Development

Learning Outcomes:

1	To comprehend the principles of histochemical techniques ;
2	To employ histochemical techniques in the laboratory and interpret the results. ;
3	To understand the principle of enzyme histochemistry and to employ these techniques in the laboratory. ;
4	To comprehend the principles and protocoles of immunohistochemical techniques ;
5	To be able to employ light and flourescence immunohistochemical techniques. ;
6	To be able to perform light and flourescence microscopic analyses in immunohistochemistry. ;
7	To quantitavely analyse the preparations resulted after immunohistochemical staining. ;
8	To comprehend the electroimmunohistochemistry techniques. ;

22	Course Content:

Week	Theoretical	Practical
1	The aim and teaching plan of the course	Overview of the histochemistry laboratory
2	Introduction to histochemical techniques	Preparation of solutions
3	Histochemical procedures	Principles of the techniques
4	Enzyme histochemistry	Enzymatic reactions
5	Enzymatic staining	Staining technique of enzyme histochemistry
6	Principles of immunohistochemistry	Overview of the immunohistochemistry laboratory
7	Immunohistochemical tissue preparation, fixation and sectioning	Laboratory: Fixation and sectioning
8	Antigen Retrieval Methods	Laboratory: Antigen Retrieval
9	Immunohistochemical methods for light microscopy	Laboratory: Immunohistochemistry for light microscopy
10	Immunohistochemical methods for fluorescence microscopy	Laboratory: Immunohistochemistry for fluorescence microscopy
11	Immunohistochemical methods for electron microscopy	Laboratory: Preembedding immunohistochemistry for electron microscopy and analysis
12	Light microscopical analysis for immunohistochemistry	Laboratory: Light microscopical analysis of the preparations
13	Fluorescence microscopical analysis for immunohistochemistry	Laboratory: Fluorescence microscopical analysis of the preparations
14	Principles of quantitative analysis of immunohistochemical stainings	Laboratory: Quantitative analysis of immunohistochemical stainings

23	Textbooks, References and/or Other Materials:	Immunocytochemistry: A Practical Approach. JE Beesley (Editor). Theory and Practice of Histological Techniques. JD
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	2	0
Final Exam	1	100
Total	3	100
Contribution of Term (Year) Learning Activities to Success Grade		0
Contribution of Final Exam to Success Grade		100
Total		100
Measurement and Evaluation Techniques Used in the Course
Information

ECTS / WORK LOAD TABLE

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High