Doç.Dr. Elif ERTÜRK Bursa Uludağ Üniversitesi, Sağlık Hizmetleri MYO, Görükle Kampüsü, Nilüfer/BURSA
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Website:
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Objective of the Course:
Teaching basic principles of molecular biology techniques to students.
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Contribution of the Course to Professional Development
Students will be provided with knowledge, skills and safe working principles about current molecular methods in molecular biology, molecular genetics laboratories.
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Learning Outcomes:
1
At the end of this lesson, the student; Will be able to explain the usage reasons of basic molecular biological methods.;
2
Will be able to Explain What are the Basic Molecular Biological Methods and Their Stages to be Used.;
3
Will be able to learn experimental design of problems based on molecular biology.;
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Will be able to follow the developments in molecular biology techniques by following the new information in the literature.;
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Course Content:
Week
Theoretical
Practical
1
General instrument introduction and molecular biology laboratory rules
2
DNA isolation and analysis
3
RNA isolation and analysis
4
Spectrophotometric methods
5
Calculation of isolated DNA / RNA / Protein concentrations
6
Agarose gel electrophoresis
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Polymerase chain reaction (PCR)
8
Agarose gel electrophoresis after polymerase chain reaction (PCR)
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RFLP method
10
Isolation and purification of proteins
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Determination of protein concentration and electrophoretic analysis
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Basic principles of enzymatic analysis and methods for determining enzyme activity
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Cell culture
14
Cell culture methods
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Textbooks, References and/or Other Materials:
1. Methods Used in Molecular Biology, Prof.Dr. Güler Temizkan, Prof. Dr. Nazlı Arda, Nobel Medical Bookstores, 2008 2. Dale, von Schantz, From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, John Wiley & Sons, Ltd, USA, 2007 (ISBN: 9780470017340) 3. Molecular Biology Techniques: An Intensive Laboratory Course (Walt Ream, Katharine G. Field), 1999.
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Assesment
TERM LEARNING ACTIVITIES
NUMBER
PERCENT
Midterm Exam
1
40
Quiz
0
0
Homeworks, Performances
0
0
Final Exam
1
60
Total
2
100
Contribution of Term (Year) Learning Activities to Success Grade
40
Contribution of Final Exam to Success Grade
60
Total
100
Measurement and Evaluation Techniques Used in the Course
Ensuring student participation in the course with homework and projects related to the subject contents
Information
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ECTS / WORK LOAD TABLE
Activites
NUMBER
TIME [Hour]
Total WorkLoad [Hour]
Theoretical
14
2
28
Practicals/Labs
0
0
0
Self Study and Preparation
0
0
0
Homeworks, Performances
0
2
30
Projects
16
2
32
Field Studies
0
0
0
Midtermexams
1
15
15
Others
0
0
0
Final Exams
1
15
15
Total WorkLoad
120
Total workload/ 30 hr
4
ECTS Credit of the Course
4
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CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
