| 1 | Course Title: | DIAGNOSTIC METHODS IN MICROBIOLOGY-I |
| 2 | Course Code: | TMK5001 |
| 3 | Type of Course: | Compulsory |
| 4 | Level of Course: | Second Cycle |
| 5 | Year of Study: | 1 |
| 6 | Semester: | 1 |
| 7 | ECTS Credits Allocated: | 5 |
| 8 | Theoretical (hour/week): | 2 |
| 9 | Practice (hour/week) : | 2 |
| 10 | Laboratory (hour/week) : | 0 |
| 11 | Prerequisites: | None |
| 12 | Recommended optional programme components: | None |
| 13 | Language: | Turkish |
| 14 | Mode of Delivery: | Face to face |
| 15 | Course Coordinator: | Doç. Dr. OKTAY ALVER |
| 16 | Course Lecturers: | Yok |
| 17 | Contactinformation of the Course Coordinator: | Doç. Dr. Oktay Alver |
| 18 | Website: | |
| 19 | Objective of the Course: | To learn about the methods used for diagnosis in microbiology and their areas of use and to make an application accordingly. |
| 20 | Contribution of the Course to Professional Development | Students will gain the knowledge and skills to be able to work or research in microbiology laboratory. |
| 21 | Learning Outcomes: |
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| 22 | Course Content: |
| Week | Theoretical | Practical |
| 1 | Main equipments used in microbiology laboratory and their features | Introducing glass materials, devices and equipment used in the microbiology laboratory |
| 2 | Working order of the microbiology laboratory | On-site examination of microbiology laboratories (Bacteriology, Serology, ELISA, PCR, Mycology, Tuberculosis, Parasitology) |
| 3 | Direct Diagnosis in Microbiology - Microscopic Diagnosis; Light Microscopy | Bacteriology: Evaluating directly stained preparations |
| 4 | Direct Diagnosis in Microbiology - Culture media used for identification of microorganisms - properties and intended use | Bacteriology: Introducing the media, inoculating techniques, evaluating the cultivated media |
| 5 | Direct Diagnosis in Microbiology - Culture | Mycology: Microscopy, macroscopy, germ tube |
| 6 | Direct Diagnosis in Microbiology - Hybridization | Parasitology: Microscopy, macroscopy, MEZN and Trichrome staining method |
| 7 | Direct Diagnosis in Microbiology - Nucleic Amplification | Tuberculosis: Microscopy; EZN staining method |
| 8 | Direct Diagnosis in Microbiology - Microscopic Diagnosis; Light Microscopy and Fluorescence Microscopy | ELISA: Macro ELISA method |
| 9 | Indirect Diagnosis in Microbiology - Precipitation, Agglutination | ELISA: Micro ELISA and ELFA technique |
| 10 | Indirect Diagnosis in Microbiology Neutralization, Complement fixation test | Serology: Aglutination, indirect hemagglutination |
| 11 | Indirect Diagnosis in Microbiology - Antigen and Antibody | Serology: Complement fixation test, Nepholometric method |
| 12 | Indirect Diagnosis in Microbiology - Enzyme Immuno Assay (EIA, ELISA, ELFA) | Serology: Precipitation, Agglutination |
| 13 | Indirect Diagnosis in Microbiology - Immunofluorescent methods | PCR: Amplification |
| 14 | Indirect Diagnosis in Microbiology - Antigen and Antibody | PCR: Real Time PCR |
| 23 | Textbooks, References and/or Other Materials: |
1. Prof. Dr. Hakkı Bilgehan. Klinik Mikrobiyolojik Tanı;, Barış Yayınları; 3. Baskı 2002. 2. Murray PR (Ed). Başustaoğlu AC (çeviri Ed.) Klinik Mikrobiyoloji. Atlas Kitapçılık; 9. Baskı. 2009. 3. Forbes BA, Sahm DF, Weıssfeld A. Bailey & Scott's Diagnostic Microbiology 11th Edition Mosby Inc. 2002. |
| 24 | Assesment |
| TERM LEARNING ACTIVITIES | NUMBER | PERCENT |
| Midterm Exam | 0 | 0 |
| Quiz | 0 | 0 |
| Homeworks, Performances | 0 | 0 |
| Final Exam | 1 | 100 |
| Total | 1 | 100 |
| Contribution of Term (Year) Learning Activities to Success Grade | 0 | |
| Contribution of Final Exam to Success Grade | 100 | |
| Total | 100 | |
| Measurement and Evaluation Techniques Used in the Course | Evaluation is made with a classical written exam. Assessment is made with a classical written exam. Assessment is carried out by classical written examination. | |
| Information | ||
| 25 | ECTS / WORK LOAD TABLE |
| Activites | NUMBER | TIME [Hour] | Total WorkLoad [Hour] |
| Theoretical | 14 | 2 | 28 |
| Practicals/Labs | 14 | 2 | 28 |
| Self Study and Preparation | 14 | 5 | 70 |
| Homeworks, Performances | 0 | 0 | 0 |
| Projects | 0 | 0 | 0 |
| Field Studies | 0 | 0 | 0 |
| Midtermexams | 0 | 0 | 0 |
| Others | 0 | 0 | 0 |
| Final Exams | 1 | 24 | 24 |
| Total WorkLoad | 150 | ||
| Total workload/ 30 hr | 5 | ||
| ECTS Credit of the Course | 5 |
| 26 | CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| LO: Learning Objectives | PQ: Program Qualifications |
| Contribution Level: | 1 Very Low | 2 Low | 3 Medium | 4 High | 5 Very High |