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COURSE SYLLABUS
RECOMBINANT DNA TECHNOLOGY AND GENETIC ENGINEERING
1 Course Title: RECOMBINANT DNA TECHNOLOGY AND GENETIC ENGINEERING
2 Course Code: MBG5436
3 Type of Course: Optional
4 Level of Course: Second Cycle
5 Year of Study: 1
6 Semester: 2
7 ECTS Credits Allocated: 6
8 Theoretical (hour/week): 3
9 Practice (hour/week) : 0
10 Laboratory (hour/week) : 0
11 Prerequisites: -
12 Recommended optional programme components: None
13 Language: Turkish
14 Mode of Delivery: Face to face
15 Course Coordinator: Prof. Dr. AYDIN TÜRKEÇ
16 Course Lecturers: Prof.Dr. Aydın Türkeç
17 Contactinformation of the Course Coordinator: Prof.Dr. Aydın Türkeç
BUÜ Fen Edebiyat Fakültesi
Moleküler Biyoloji ve Genetik Bölümü
Kat:1 no:110
Tel: 02242942861
E:posta: aturkec@uludag.edu.tr
18 Website:
19 Objective of the Course: Aim of this Course is to familiarize students with the most frequently used methods in Recombinant DNA Technology and Genetic Engineering from both a theoretical and experimental point of view
20 Contribution of the Course to Professional Development At the end of this course students gain the competence to prepare projects and to strive to meet the country’s requirements by using recombinant DNA methods.
21 Learning Outcomes:
1 At the end of this course students learn basic methods of gene cloning and manipulation for protein expression.;
2 At the end of this course students learn how recombinant DNA technology is used in genetic engineering to modify prokaryotic and eukaryotic cells;
3 Students gain information about practical and biotechnological applications of recombinant DNA technology.;
22 Course Content:
Week Theoretical Practical
1 Recombinant DNA Technology and Genetic Engineering and Their Applications
2 DNA Restiction Enzymes
3 Cloning and Expression Vectors
4 Gel Elecrophoresis of DNA and Proteins and Hybridization Techniques
5 Gene Transfer Techniques
6 DNA Sequence Analysis
7 Polymerase Chain Reaction
8 Construction and Use of Genomic and cDNA Libraries
9 Manipulating DNA in Microorganisms other than E. coli
10 Production of Recombinant Proteins
11 Production of Vaccines and Hormones in Microorganisms
12 Use of Transgenic Plants
13 Transgenic Animals and their Applications
14 Impacts of Recombinant DNA and Genetic Engineering on the Biological Sciences, Medicine and Industry
23 Textbooks, References and/or Other Materials:
24 Assesment
TERM LEARNING ACTIVITIES NUMBER PERCENT
Midterm Exam 1 40
Quiz 0 0
Homeworks, Performances 0 0
Final Exam 1 60
Total 2 100
Contribution of Term (Year) Learning Activities to Success Grade 40
Contribution of Final Exam to Success Grade 60
Total 100
Measurement and Evaluation Techniques Used in the Course The system of relative evaluation is applied
Information
25 ECTS / WORK LOAD TABLE
Activites NUMBER TIME [Hour] Total WorkLoad [Hour]
Theoretical 14 3 42
Practicals/Labs 0 0 0
Self Study and Preparation 3 14 42
Homeworks, Performances 0 7 28
Projects 0 0 0
Field Studies 0 0 0
Midtermexams 1 25 25
Others 1 18 18
Final Exams 1 25 25
Total WorkLoad 180
Total workload/ 30 hr 6
ECTS Credit of the Course 6
26 CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
PQ1 PQ2 PQ3 PQ4 PQ5 PQ6 PQ7 PQ8 PQ9 PQ10
LO1 5 5 5 5 5 4 4 4 4 4
LO2 3 4 4 4 5 4 4 5 4 5
LO3 4 4 5 4 4 4 5 3 4 5
LO: Learning Objectives PQ: Program Qualifications
Contribution Level: 1 Very Low 2 Low 3 Medium 4 High 5 Very High
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