Tel: 0224 2941266 E-mail: tilhan@uludag.edu.tr Bursa Uludağ Üniversitesi Veteriner Fakültesi A Blok Histoloji-Embriyoloji A.D.
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Website:
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Objective of the Course:
Teaching the most used molecular techniques in the histology laboratory.
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Contribution of the Course to Professional Development
To learn the molecular techniques that can be used in scientific laboratory studies in the field of histology and to reach the competence to apply.
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Learning Outcomes:
1
To be able to make calculations for laboratory applications.;
2
To be able to apply the techniques of purification of macromolecules, gel electrophoresis and spectrophotometric analysis on their own.;
3
To gain the ability to use histological methods and molecular techniques in a laboratory.;
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Course Content:
Week
Theoretical
Practical
1
Safety in the molecular biology laboratory; Presentation of laboratory and laboratory equipments.
2
Micropipette use, Laboratory calculations; pH adjustments; Preparation of solutions and buffers to be used.
3
Separation and purification of macromolecules.
4
Agarose gel electrophoresis.
5
PCR (Working principle, optimization)
6
PCR (Application areas, application).
7
Marking of proteins with flourescence.
8
Observation of fluorescently labeled proteins by microscope.
9
Types of blotting (Western, Southern)
10
Western Blot (Collection of tissue protein, calculation)
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Western Blot (gel preparation, loading)
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Western Blot (Blotting, Viewing)
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ELISA (General principles, calculations).
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ELISA (Application of the method).
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Textbooks, References and/or Other Materials:
Dale, von Schantz, From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, John Wiley & Sons, Ltd, USA, 2007 (ISBN: 9780470017340) At the banch,Barker K.,Cold Spring Harbor Laboratory Press, New York,1998.
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Assesment
TERM LEARNING ACTIVITIES
NUMBER
PERCENT
Midterm Exam
0
0
Quiz
0
0
Homeworks, Performances
0
0
Final Exam
1
100
Total
1
100
Contribution of Term (Year) Learning Activities to Success Grade
0
Contribution of Final Exam to Success Grade
100
Total
100
Measurement and Evaluation Techniques Used in the Course
The evaluation will be done with open-ended questions, short answer questions and multiple choice questions.
Information
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ECTS / WORK LOAD TABLE
Activites
NUMBER
TIME [Hour]
Total WorkLoad [Hour]
Theoretical
14
2
28
Practicals/Labs
0
0
0
Self Study and Preparation
14
4
56
Homeworks, Performances
0
0
0
Projects
0
0
0
Field Studies
0
0
0
Midtermexams
0
0
0
Others
0
0
0
Final Exams
1
1
1
Total WorkLoad
85
Total workload/ 30 hr
2,83
ECTS Credit of the Course
3
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CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
