COURSE SYLLABUS

ANIMAL CELL CULTURE

1	Course Title:	ANIMAL CELL CULTURE
2	Course Code:	BIO5402
3	Type of Course:	Optional
4	Level of Course:	Second Cycle
5	Year of Study:	1
6	Semester:	2
7	ECTS Credits Allocated:	6
8	Theoretical (hour/week):	3
9	Practice (hour/week) :	0
10	Laboratory (hour/week) :	0
11	Prerequisites:	None
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. FERDA ARI
16	Course Lecturers:
17	Contactinformation of the Course Coordinator:	Prof Dr. Ferda ARI Bursa Uludağ Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü 16059 Nilüfer/BURSA Tlf: 0 224 294 1822 e-posta: ferdaoz@uludag.edu.tr
18	Website:
19	Objective of the Course:	The aim and objectives of the course is studying the features of tissue and cell cultures, preparing cell cultures, special techniques and applications
20	Contribution of the Course to Professional Development	The lessons learned in this course will enable students to apply cell techniques in laboratories such as molecular biology, biochemistry and genetics where they can work.

Learning Outcomes:

1	Able to learn and apply the principles of cell culture;
2	Understanding the needs of the cell culture and equipment;
3	Determine the viability of cells in different ways;
4	Understand cell death pathways;
5	Comprehend gene and blotting analysis in cell culture;

22	Course Content:

Week	Theoretical	Practical
1	Tissue and cell culture, basic definitions and concepts
2	Labrotuvar properties of cell culture, introducing the necessary equipment and materials.
3	The use of laminar flow cabinet, the use of CO2 incubator, regulation of the cell culture chamber
4	General techniques in cell culture: Definition of cell types
5	Contamination and Sterilization in Cell Cultures
6	Cell freezing and thawing techniques
7	Cell counting methods, planting methods and the passage
8	Cell counting and cultivation methods
9	Viability tests in cells SRB, MTT, XTT test ATP and Trypan blue tests
10	Analyzes for the Determination of Apotosis in Cell Culture
11	Analysis for Determination of Necrosis in Cell Culture
12	Analysis for the Determination of Autophagy in Cell Culture
13	Gene analysis in Cell Culture
14	Western Blot analysis methods in Cell Culture

23	Textbooks, References and/or Other Materials:	Recent papers Introduction to Cell and Tissue Culture: Theory and Technique (Jennie P. Mather, Penelope E. Roberts ) Cell Culture Techniques (Editors: Aschner, Michael, Suñol, Cristina, Bal-Price, Anna (Eds.) Doku ve Hücre Kültürü Teknikleri (İsmail Kocaçalışkan)
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	1	40
Final Exam	1	60
Total	2	100
Contribution of Term (Year) Learning Activities to Success Grade		40
Contribution of Final Exam to Success Grade		60
Total		100
Measurement and Evaluation Techniques Used in the Course	The system of relative evaluation is applied
Information	The system of relative evaluation is applied

ECTS / WORK LOAD TABLE

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High