RECOMBINANT DNA TECHNOLOGY AND GENETIC ENGINEERING
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Course Title:
RECOMBINANT DNA TECHNOLOGY AND GENETIC ENGINEERING
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Course Code:
MBG5436
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Type of Course:
Optional
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Level of Course:
Second Cycle
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Year of Study:
1
6
Semester:
2
7
ECTS Credits Allocated:
6
8
Theoretical (hour/week):
3
9
Practice (hour/week) :
0
10
Laboratory (hour/week) :
0
11
Prerequisites:
-
12
Recommended optional programme components:
None
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Language:
Turkish
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Mode of Delivery:
Face to face
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Course Coordinator:
Prof. Dr. AYDIN TÜRKEÇ
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Course Lecturers:
Prof. Dr. Aydın Türkeç
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Contactinformation of the Course Coordinator:
Prof. Dr. Aydın Türkeç B.U.Ü. Moleküler Biyoloji ve Genetik Bölümü aturkec@uludag.edu.tr 02242942861
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Website:
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Objective of the Course:
Aim of this Course is to familiarize students with the most frequently used methods in Recombinant DNA Technology and Genetic Engineering from both a theoretical and experimental point of view, to illustrate creative use of modern tools and techniques for manipulation and analysis of genomic sequences, to expose students to application of recombinant DNA technology in biotechnological research, to train students in strategizing research methodologies employing genetic engineering techniques.
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Contribution of the Course to Professional Development
Student will have learned recombinant DNA technolohgy and genetic engineering and will be able to apply them in experiments
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Learning Outcomes:
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At the end of this course students learn basic methods of recombinant DNA and manipulation in biotechnological researches;
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At the end of this course students learn how recombinant DNA technology is used in genetic engineering to modify organisms;
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Students gain information about practical and biotechnological applications of recombinant DNA technology and application of genetic engineering techniques in basic and applied experimental biology;
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At the end of this course students gain the skills proficiency in designing and conducting experiments involving rekcomninatn DNA and genetic manipulation and ability to discuss in ethical perspective;
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At the end of this course students learn the principles of advanced techniques in recombinant DNA technology and interpretation of results obtained by these techniques and adapt these data to everyday life;
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Course Content:
Week
Theoretical
Practical
1
Introduction to recombinant DNA technology
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Basic techniques in recombinant DNA technology
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Gene cloning strategies
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Gene cloning and expression vectors
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Analyses of gene expression and function
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Genomic and cDNA libraries
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Manipulating DNA in Microorganisms other than E. coli
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Genome mapping
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Methods for genetic transformation
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Application of genetic engineering
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Production of recombinant proteins
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Transgenic plants and animals
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Genetically modifeied organisms and GMO analysis methods
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Effects of recombinatn DNA Technology and genetic engineering on biological sicence and biotechnology and principle of bioethics and biosafety
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Textbooks, References and/or Other Materials:
Principles of Gene Manipulation Sandy B., Primrose, Richard M. Twyman, Madlen, MA; Oxford: Blackwell Pub., (2006) Gene Cloning and DNA Analysis An Introduction Fourth Edition T.A Brown An Introduction to Genetic Engineering (Studies in Biology) (2002) Desmond S. T. Nicholl Cambridge University Press From Genes to Genomes: Concepts and Applicaitons of DNA Technology, 2nd Edition, Dale, von Schantz, John Wiley & Sons, Ltd, ISBN: 978-0-470-01734-0, Biotechnology An Introduction S. Ignacimuthi, ISBN- 13:978-1842657546 Biotechnology for Biomedical Applications,Bronzino, ISBN : 0849318114
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Assesment
TERM LEARNING ACTIVITIES
NUMBER
PERCENT
Midterm Exam
1
40
Quiz
0
0
Homeworks, Performances
0
0
Final Exam
1
60
Total
2
100
Contribution of Term (Year) Learning Activities to Success Grade
40
Contribution of Final Exam to Success Grade
60
Total
100
Measurement and Evaluation Techniques Used in the Course
The system of relative evaluation is applied
Information
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ECTS / WORK LOAD TABLE
Activites
NUMBER
TIME [Hour]
Total WorkLoad [Hour]
Theoretical
14
3
42
Practicals/Labs
0
0
0
Self Study and Preparation
3
14
42
Homeworks, Performances
0
7
28
Projects
0
0
0
Field Studies
0
0
0
Midtermexams
1
25
25
Others
1
18
18
Final Exams
1
25
25
Total WorkLoad
180
Total workload/ 30 hr
6
ECTS Credit of the Course
6
26
CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
