Prof Dr. Ferda ARI Bursa Uludağ Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü 16059 Nilüfer/BURSA Tlf: 0 224 294 1822 e-posta: ferdaoz@uludag.edu.tr
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Website:
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Objective of the Course:
The aim of this course is to increase students' basic principles of methods commonly used in molecular biology and knowledge about equipment and to teach laboratory conditions.
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Contribution of the Course to Professional Development
This course will provide students with the opportunity to apply the necessary knowledge and molecular techniques in areas such as molecular biology, biochemistry and genetics.
Understands the disintegration and purification of macromolecules;
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Understand the application of spectrophotometric methods;
4
Understands chromatographic methods;
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Understand the isolation and working principles of nucleic acids such as DNA and RNA;
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Understand PCR and gene analysis;
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Comprehend ELISA and blotting methods;
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Understand epigenetic analysis;
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Course Content:
Week
Theoretical
Practical
1
Molecular biology basic laboratory rules and laboratory equipment
2
Basic Macromolecules
3
Fragmentation Methods used in Molecular Biology
4
Separation Methods Used in Molecular Biology
5
Spectrophotometric Methods
6
Chromatographic methods I
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Chromatographic methods II
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Purification and isolation of macromolecules
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Enzymatic Analysis
10
ELISA and blotting techniques
11
Cell Culture and its usage in molecular biology
12
Analysis methods and marking of nucleic acids
13
PCR and gene analysis methods
14
Epigenetic analysis
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Textbooks, References and/or Other Materials:
Basic Methods in Molecular Biology (Leonard G. Davis, Mark D. Dibner and James F. Battey) • Analytical Techniques in Biochemistry and Molecular Biology (Rajan Katoch) • General Techniques of Cell Culture (Maureen A. Harrison) • Current reviews and publications
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Assesment
TERM LEARNING ACTIVITIES
NUMBER
PERCENT
Midterm Exam
0
0
Quiz
0
0
Homeworks, Performances
1
40
Final Exam
1
60
Total
2
100
Contribution of Term (Year) Learning Activities to Success Grade
40
Contribution of Final Exam to Success Grade
60
Total
100
Measurement and Evaluation Techniques Used in the Course
The system of relative evaluation is applied
Information
The system of relative evaluation is applied
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ECTS / WORK LOAD TABLE
Activites
NUMBER
TIME [Hour]
Total WorkLoad [Hour]
Theoretical
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3
42
Practicals/Labs
0
0
0
Self Study and Preparation
14
6
84
Homeworks, Performances
1
15
45
Projects
0
0
0
Field Studies
0
0
0
Midtermexams
0
0
0
Others
0
0
0
Final Exams
1
10
10
Total WorkLoad
181
Total workload/ 30 hr
6,03
ECTS Credit of the Course
6
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CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
