COURSE SYLLABUS

MOLECULAR BIOLOGY TECHNIQUES

1	Course Title:	MOLECULAR BIOLOGY TECHNIQUES
2	Course Code:	MBG2006
3	Type of Course:	Compulsory
4	Level of Course:	First Cycle
5	Year of Study:	2
6	Semester:	4
7	ECTS Credits Allocated:	6
8	Theoretical (hour/week):	2
9	Practice (hour/week) :	2
10	Laboratory (hour/week) :	0
11	Prerequisites:	None
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Dr. Ögr. Üyesi ELİF UZ
16	Course Lecturers:	Araştırma Görevlileri
17	Contactinformation of the Course Coordinator:	Dr. Öğr. Üyesi Elif UZ YILDIRIM Bursa Uludağ Üniversitesi Fen-Edebiyat Fak., Moleküler Biyoloji ve Genetik Bölümü elifuz@uludag.edu.tr 902242941776
18	Website:
19	Objective of the Course:	To comprehend the basic logic of the techniques used in molecular biology and genetics studies.
20	Contribution of the Course to Professional Development	To gain the capacity of choosing the right method during experimental studies. Moreover, to choose the right techiques, since the students learn the similarities and differences among methods.

Learning Outcomes:

1	Learn the basic methods used in molecular biology.;
2	Gain the capacity to design their own experiments;
3	Learn basic equipments and consumables used in a molecular biology laboratory.;
4	Gain the basic skills in order to work in a molecular biology laboratory.;
5	Comprehend how to follow novel developments in the techniques used in molecular biology.;

22	Course Content:

Week	Theoretical	Practical
1	Basic concepts of techniques used in molecular biology: A general overview I	Laboratory rules and safety (Groups A1-B1)
2	Basic concepts of techniques used in molecular biology: A general overview II	Laboratory rules and safety (Groups A2-B2)
3	Isolation techniques (DNA, RNA and protein)	Laboratory equipments (Groups A1-B1)
4	cDNA synthesis, library preparation	Laboratory equipments (Groups A2-B2)
5	Conepts of PCR	Laboratory consumables (Groups A1-B1)
6	Types of PCR and concepts of RT-PCR	Laboratory consumables (Groups A2-B2)
7	Electrophoretic techniques (Agarose and PAGE)	Solution Preparation (Groups A1-B1)
8	Principles of hybridization techniques (Southern and Northern blot)	Solution Preparation (Groups A2-B2)
9	Principles of hybridization techniques (Western blot)	DNA Isolation (Groups A1-B1)
10	DNA sequencing techniques	DNA Isolation (Groups A2-B2)
11	siRNA/miRNA, Microarray	Spectrophotometric measurements of DNA and Agarose Gel (Groups A1-B1)
12	Genome editing using nucleases (ZFNs, TALENs and CRISPR-Cas)	Spectrophotometric measurements of DNA and Agarose Gel (Groups A2-B2)
13	ChIP, ELISA, protein sequencing	PCR and gel electrophoresis (Groups A1-B1)
14	Yeast complementation: Yeast one hybrid, yeast two hybrid and yeast three hybrid	PCR and gel electrophoresis (Groups A2-B2)

23	Textbooks, References and/or Other Materials:	Temel ve İleri Moleküler Biyoloji Yöntemleri-Genomik ve Proteomik Analizler, Güler Temizkan, Nazlı Arda, Nobel Tıp Kitabevi, 2017.
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	1	25
Quiz	6	15
Homeworks, Performances	0	0
Final Exam	1	60
Total	8	100
Contribution of Term (Year) Learning Activities to Success Grade		40
Contribution of Final Exam to Success Grade		60
Total		100
Measurement and Evaluation Techniques Used in the Course	Midterm and final Exam questions. Moreover, lab quizes, lab reports and attendence to lab courses.
Information	Measurement and evaluation is perfomed by relative evaluation system

ECTS / WORK LOAD TABLE

CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High