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Course Title: |
GENETIC |
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Course Code: |
VET1019 |
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Type of Course: |
Compulsory |
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Level of Course: |
First Cycle |
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Year of Study: |
1 |
| 6 |
Semester: |
1 |
| 7 |
ECTS Credits Allocated: |
2 |
| 8 |
Theoretical (hour/week): |
2 |
| 9 |
Practice (hour/week) : |
0 |
| 10 |
Laboratory (hour/week) : |
0 |
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Prerequisites: |
None |
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Recommended optional programme components: |
None |
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Language: |
English |
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Mode of Delivery: |
Face to face |
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Course Coordinator: |
Doç.Dr. ÖZDEN ÇOBANOĞLU |
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Course Lecturers: |
Doç. Dr. Özden ÇOBANOĞLU
Doç. Dr. Sena ARDIÇLI
Araş. Gör. Dr. Deniz DİNÇEL
|
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Contactinformation of the Course Coordinator: |
Doç. Dr. Özden ÇOBANOĞLU
U.Ü. Veteriner Fakültesi Genetik Anabilim Dalı Nilüfer/BURSA
E-mail:ocobanoglu@uludag.edu.tr
Tel: 0 224 294 1241
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Website: |
http://www.veteriner.uludag.edu.tr |
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Objective of the Course: |
This course covers principles of prokaryotic and eukaryotic genetics. In this course, students will expand on the basic knowledge of genetics. This will involve learning new terminology and new core concepts about the principle of genetics which will be the basis for the other classes during their education. They will able to apply the general concept of genetics to veterinary science. The molecular basis of heredity, chromosome structure, patterns of Mendelian and non-Mendelian inheritance, and biotechnological applications will be covered in this course. Thus, the course provides the students with a review of analytical, molecular and cellular genetics along with new developments. Upon successful completion of this course, students should be able to recognize and describe genetic phenomena and demonstrate knowledge of important genetic principles. |
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Contribution of the Course to Professional Development |
The course will provide the students with the necessary knowledge about the basic concepts related to the genetic structure of biological creatures during their further education. |
| Week |
Theoretical |
Practical |
| 1 |
Introduction to the Course and Milestones of Genetics; Mendelian Genetics:
The chromosomal basis of inheritance, Mendel’s principles of segregation, and independent assortment, monohybrid, dihybrid and trihybrid crosses. |
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| 2 |
Variations on Mendelian Inheritance I:
Genetic deviations from Mendelian principles, and examples of non-Mendelian inheritance; like incomplete dominance, co-dominance, gene interactions, and type of epistasis. |
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| 3 |
Exceptions on Mendelian Genetics II:
Examination of other examples for the mode of non-Mendelian inheritance, like pleiotropy, multiple alleles, polygenic inheritance, essential genes, lethal alleles, penetrance, environmental effects, and genetic heterogeneity. |
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Sex Linked Inheritance:
Sex determination in different species, sex linkage, X chromosome Inactivation, dosage compensation, cytoplasmic inheritance, genetic maternal effect, sex-influenced characteristics and pedigree analysis. |
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Linkage and Chromosomal Mapping:
Linkage and recombination, crossing over, chromosome theory, a genetic map of the Drosophila melanogaster, linkage mapping in dihybrid and trihybrid cross by recombination frequencies between genes, interference, and coefficient of coincidence. |
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Identifying DNA and RNA as the Genetic Material:
Search for genetic material; the discovery of DNA by Griffith’s Transformation Experiment, Avery, MacLeod and McCarty’s experiments, Hershey-Chase bacteriophage experiment, and a discovery of RNA by Tobacco Mosaic Virus (TMV) experiment. |
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| 7 |
The Structure and Analysis of DNA and RNA:
Structure of nucleic acid, properties of pyrimidines and purines, the anatomy of DNA, a discovery of the structure of DNA, the DNA double helix as Watson and Crick model, polymorphism of DNA, structural features of DNA and a structure of RNA. |
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| 8 |
DNA Packing in Prokaryotic and Eukaryotic Chromosomes:
DNA condensation, DNA supercoiling, nucleosomes, eukaryotic chromosomal organization, a structure of chromatin, chromosome folding, DNA packing.
Gene Expression and Regulation:
Repressible vs. inducible operon systems; Lac Operon and Tryptophan Operons in E. coli. |
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DNA Replication in Prokaryotes and Eukaryotes:
Models for DNA replication, Meselson-Stahl experiment, a mechanism of DNA replication in prokaryotes, replication of DNA in eukaryotes, enzymes required for replication, directionality of synthesis in DNA strands, DNA repair system, editing, and proofreading of DNA. |
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The Central Dogma; Transcription, Translation and Protein Synthesis:
Defining central dogma of molecular biology, transcription, RNA processing, genetic code, wobble base pairing, translation, protein synthesis, the structure of amino acid, principles of polarity in amino acid. |
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The Genetic Mutations:
Cause of mutation, types of mutations; spontaneous mutations, single base substitution and frameshift mutations, chromosomal disorders, nondisjunction in autosomal chromosomes, trisomies, nondisjunction of X chromosomes and induced mutations. |
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Recombinant DNA Technology:
Genetic Transfer in Bacteria: Transformation, transduction, and conjugation, plasmid structure in bacteria.
Type of vectors, techniques of recombinant DNA technology; electroporation, protoplast fusion, and injection: gene gun and microinjection. |
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DNA Sequencing Techniques:
Basic methods for sequencing; Maxam-Gilbert and Sanger methods, Whole genome sequencing and New DNA sequencing methods. |
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| 14 |
Basic Molecular Techniques:
Polymerase Chain Reaction (PCR) and Its Steps and Application; Gel Electrophoresis System, Restriction Edonuclease; RFLP, AFLP, RAPD, Microsatellite and SNP Marker Analyzes, Microarray System and Marker Assisted Selection and Use of markers in Livestock. |
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