1	Course Title:	CELL CULTURE
2	Course Code:	TİM5011
3	Type of Course:	Optional
4	Level of Course:	Second Cycle
5	Year of Study:	1
6	Semester:	1
7	ECTS Credits Allocated:	6
8	Theoretical (hour/week):	1
9	Practice (hour/week) :	4
10	Laboratory (hour/week) :	0
11	Prerequisites:	None
12	Recommended optional programme components:	None
13	Language:	Turkish
14	Mode of Delivery:	Face to face
15	Course Coordinator:	Prof. Dr. ARZU YILMAZTEPE ORAL
16	Course Lecturers:	Prof. Dr. H. Barbaros Oral
17	Contactinformation of the Course Coordinator:	Uludağ Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, 16059, Nilüfer, BURSA E-mail: arzuy@uludag.edu.tr Tel: 2953921
18	Website:
19	Objective of the Course:	The aim of this course is to provide the student knowledge and skills required for performing cell culture methods.
20	Contribution of the Course to Professional Development

21

Learning Outcomes:

1 To know the biological features of primary cells and cell lines ; 2 To comprehend the equipment, consumables and nvironment ; 3 To gain knowledge about the maintenance and storage of cell culture;

22	Course Content:

Week	Theoretical	Practical
1	Cell culture laboratory and its equipments
2	Safety in cell culture laboratories and asepsis techniques
3	Features of culture environment
4	Primary cell culture and features
5	Cell counting and viability tests
6	Suspension cell cultures and features
7	Passage in monolayer cultures and its maintenance
8	Detection of contamination and measures
9	Cell freezing and storage
10	Specific cell separation methods
11	Flow cytometry in cell culture
12	Immunochemical staining in cell culture
13	Cell-based ELISA
14	Fluorescent plate tests used in cell culture

23	Textbooks, References and/or Other Materials:	1. Thompson L., "Measuring Immunity: Basic and clinical practice", Elsevier Academi,c Press (2005). 2. Buyru N., Dalay N., Özgüç M., Öztürk M., Sakızlı, M. (Çeviri editörleri) "Hücrenin Moleküler Biyolojisi", TÜBA Yayınları, 1. Basım (2008) 3. Green M., Sambrook J., "Molecular Cloning: A Laboratory Manual", Cold Springer Harbor Laboratory Press (2012)
24	Assesment

TERM LEARNING ACTIVITIES	NUMBER	PERCENT
Midterm Exam	0	0
Quiz	0	0
Homeworks, Performances	5	50
Final Exam	1	50
Total	6	100
Contribution of Term (Year) Learning Activities to Success Grade		50
Contribution of Final Exam to Success Grade		50
Total		100
Measurement and Evaluation Techniques Used in the Course
Information

25

ECTS / WORK LOAD TABLE

Activites	NUMBER	TIME [Hour]	Total WorkLoad [Hour]
Theoretical	14	1	14
Practicals/Labs	14	4	56
Self Study and Preparation	14	4	56
Homeworks, Performances	5	10	50
Projects	0	0	0
Field Studies	0	0	0
Midtermexams	0	0	0
Others	0	0	0
Final Exams	1	4	4
Total WorkLoad			180
Total workload/ 30 hr			6
ECTS Credit of the Course			6

26	CONTRIBUTION OF LEARNING OUTCOMES TO PROGRAMME QUALIFICATIONS
PQ1 PQ2 PQ3 PQ4 PQ5 PQ6 PQ7 LO1 0 0 5 0 0 0 0 LO2 0 0 5 0 0 0 0 LO3 0 0 5 0 0 0 0

LO: Learning Objectives

PQ: Program Qualifications

Contribution Level:

1 Very Low

2 Low

3 Medium

4 High

5 Very High