| Week |
Theoretical |
Practical |
| 1 |
Instruction of the contents of the course, methods of teaching and about activities during semester. Scope of microbiology and nature of microorganisms
|
General information about environmental microbiology laboratory and the rules during the laboratory study. Presentation of instruments and equipment used in environmental microbiology laboratory. Introducing the microscope. Types of microscope. Informing the students about slide preparation procedures. |
| 2 |
Structural organisations of microorganisms, description of procaryotic and eucaryotic cells. Structure of procaryotic cells. Structure of eucaryotic cells. Classification of procaryotic and eukaryotic cells and structure of bacteria |
Yeast, fungi and algae. Examination of their structure under microscope.
|
| 3 |
Classification of bacteria. Structure of algae, protozoa, fungi and viruses, metazoa and higher organisms. Chemical compositions of the cells.
|
Examination of microorganism by staining and preparation of smear (slide). Pre-applications before staining, and type of stains. Staining methods. Simple staining, differential staining, gram staining, negative staining and endospore staining. Examination of bacteria by using Gram staining method.
|
| 4 |
Microbial growth. Mathematical definition of microbial growth. Factors affecting microbial growth. Continuous cultures..
|
Culture media. Media specifications. Preparation of culture media. Microbial growth on nutrient medium. Conservation of microorganisms. Pure culture techniques. The formation of bacterial growth curve.
|
| 5 |
CEnzymes. Classification of enzymes. Enzyme reactivity and inhibition. Effect of pH and temperature on enzyme activity.
|
Sampling for microbiological tests and examination. Sterilization and disinfection techniques.
|
| 6 |
Microbial energy production and conservation. Oxidation-reduction potential, electron tower. ATP formation mechanism. assimilative and dissimilative metabolizm. Metabolic pathways, respiration, crebs cycle, oxidative phosphorylation, chemiosmosis, fermentation.
|
Dilution method and pour plate method. Heterotrophic plate count (HPC) method in drinking water.
|
| 7 |
Energy utilization. Energy for biosynthesis and maintenance. Biosynthesis. Calvin cycle. Metabolic diversity.
|
Multiple-tube method and problem solutions. |
| 8 |
Public health microbiology. Pathogenic and indicator microorganisms. |
Estimating the number of coliform by multiple-tube method. |
| 9 |
Microbiological composition of domestic wastewater and its effects on public health.
|
Estimating the number of coliform by multiple-tube method. |
| 10 |
Disinfection of water and wastewater. Factors affecting disinfection. Methods of disinfection: Chlorine, chlorine dioxide, ozone, UV radiation, photoinactivation methods
|
Membrane filtration method. Estimating the number of bacteria by membrane filtration method in dirinking water.
|
| 11 |
Drinking water microbiology. Drinking water treatment processes and drinking water distribution systems. Other biological problems and solutions related to drinking water treatment and distribution.
|
Estimating the number of E.coli in sludge by pour plate method. |
| 12 |
Activated sludge process. Nitrogen, phosphorus, sulfur cycle, Nutrient removal. Suspended growth. Nitrification and denitrification in bioreactors.
|
Spread plate count. Application of laboratory experimental design.
|
| 13 |
Bulking and foaming in activated sludge plants. The factors that cause filamentous bulking. Control of bulking and foaming. Filamentous bacteria and problems encountered in activated sludge plants.
|
Examination of activated sludge sample under microscope. Determination of sludge volume index. Application of laboratory experimental design.
|
| 14 |
Sludge microbiology. Sludge processing. Pathogen removal during sludge treatment.
|
Laboratory: Application of laboratory experimental design.
|
